盐胁迫对白三叶叶肉细胞超微结构的影响  

Effect of salt stress on ultrastructure of mesophyll cells in white clover

作　　者：殷秀杰[1] 胡宝忠[2] 崔国文[1] 李凤兰[2] 李景欣[1] 王明君[1] 洪锐民[1] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2011年第4期125-128,共4页Journal of Northeast Agricultural University

基　　金：中国博士后科学基金

摘　　要：利用透射电镜技术对盐胁迫下白三叶叶肉细胞的超微结构进行了观察。结果表明,对照组叶肉细胞排列疏松,各种细胞器结构完整,叶绿体含少量淀粉粒和脂质球。轻度盐胁迫对叶肉细胞超微结构影响较小。中度和重度盐胁迫引起叶肉细胞超微结构的改变,对叶绿体的膜系统产生伤害,叶绿体由原来的长椭圆形变成圆球状,片层逐渐解体,外形轮廓发生变化,内部片层发生轻微膨胀,基粒片层数目减少,出现大量的淀粉粒;线粒体肿胀,嵴消失,细胞内部结构趋向简单,以至降解。The ultrastructural changes of mesophyll cells during salt stress were observed in white clover（Trifolium repens L.） using transmission electron microscopy.The results showed that mesophyll cells arranged loosely and various organelles structure was integrated,at the same time,some starch grains and lipid globules were presented in chloroplasts in the control group.The influence of mild salt stress on msesophyll cell ultrastructure was less.But moderate and severe salt stress induced ultrastructure changes in mesophyll cells.Chloroplast membrane system produced injury;chloroplasts changed from the long oval into around ball;the layers gradual disintegrated,and its contour changed;the minor internal layers happened light expand;the number of grana lamella reduced,and a large number of starch granules appeared;mitochondrial cristae disappearance and mitochondrial swelling,the cell internal structure changed simplify,and even degradation.

关 键 词：白三叶 盐胁迫 叶肉细胞 超微结构 

分 类 号：S688.4[农业科学—观赏园艺] Q944.52[农业科学—园艺学]