靶向巨噬细胞表达抗菌肽PR39基因抗胞内结核分枝杆菌的研究  

Inhibitory effect of macrophage-specific expression of antimicrobial peptide PR-39 on intracellular growth of BCG in mouse macrophage cells and lung

作　　者：夏乾峰[1] 文阳安[2] 涂植光[3] 钱士匀[1] 覃西[1] 

机构地区：[1]海南医学院热带医学与检验医学院,海南海口571101 [2]重庆医科大学附属第一医院检验科 [3]重庆医科大学医学检验系,重庆400016

出　　处：《海南医学院学报》2011年第3期292-295,308,共5页Journal of Hainan Medical University

基　　金：海南省自然科学基金资助(309401)~~

摘　　要：目的:观察靶向巨噬细胞表达抗菌肽PR39基因的腺病毒在小鼠巨噬细胞RAW264.7和小鼠肺部的表达及抗结核分枝杆菌活性。方法:利用携巨噬细胞启动子和抗菌肽PR39基因的重组腺病毒感染体外培养的小鼠巨噬细胞RAW264.7和小鼠肺部,采用荧光免疫技术检测PR39在小鼠肺部表达,并对其抗菌活性进行初步检测。结果:重组腺病毒能够成功感染RAW264.7,并有效表达抗菌肽PR39。与对照相比,感染重组腺病毒的RAW264.7具有更强的杀灭胞内BCG的能力,同时,重组腺病毒能够成功感染小鼠肺部,并表达目的基因。与对照相比,感染重组腺病毒的小鼠肺部的BCG活菌数明显减少。结论:靶向巨噬细胞表达抗菌肽PR39基因在体内外均能够发挥抗胞内BCG作用,为抗菌肽PR39在胞内菌感染基因治疗中的应用奠定了实验基础。Objective：To construct an adenovirus expression vector encoding antimicrobial peptide PR39,and to observe its expression and antimicrobial activity in mouse macrophage RAW264.7 cells and lung.Methods：Recombinant adenovirus vector pAd-PR39 was used to infect mouse macrophage RAW264.7 cells and lungs and its antimicrobial activity against BCG was detected.The expression of PR39 gene in mouse macrophage RAW264.7 cells and lungs was detected by immunocytochemistry.Results：Recombinant adenovirus Ad-PR39 was infected into macrophage RAW264.7 cells and lung for stable or transient expression of PR-39.PR-39 expression in macrophage cells was subsequently confirmed by immunocytochemistry and showed stronger antibacterial ability against BCG than control group.Conclusions：These results demonstrate that macrophage-specific expression of antimicrobial peptide PR-39 in macrophages can inhibit the growth of intracellular BCG and it is a novel and promising approach for the control of refractory intracellular infection.

关 键 词：抗菌肽 PR39 腺病毒载体 胞内菌 BCG 巨噬细胞 

分 类 号：R346[医药卫生—基础医学]