新疆断裂带含硫冷泉泉水细菌群落结构的动态变化  

Dynamic Changes of Bacteria Community Structure in a Sulfur Cold Spring in Xinjiang Fault Zone

作　　者：李慧[1,2] 曾军[2] 高小其[3] 张涛[2] 杨晓芳[3] 孙建[2] 娄恺[2] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]新疆农业科学院微生物应用研究所,乌鲁木齐830091 [3]新疆维吾尔自治区地震局,乌鲁木齐830011

出　　处：《新疆农业科学》2011年第4期724-727,共4页Xinjiang Agricultural Sciences

基　　金：新疆特殊环境微生物实验室开放课题(XJYS0203-2008-02)

摘　　要：【目的】了解新疆断裂带10号含硫冷泉泉水中细菌群落结构的动态变化。【方法】采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)的方法,反映和分析10号冷泉泉水中细菌种群的16S rRNA基因V3区多样性及其种群基因组成随时间的变化。【结果】细菌种群的丰度(Shannon-Wiener指数(H′))和优势度(Simpson指数(D))随时间的变化均出现显著不规律的变化,且当细菌种群丰度较大时,优势度反而较小;由样品Sorenson相似距离系数形成的二维图谱中,不存在位置完全重合的样品。【结论】新疆断裂带10号含硫冷泉泉水中细菌群落结构表现出显著随机的动态变化。【Objective】This present study researched the dynamic change of the bacteria community over time in the No.10 cold spring in Xinjiang faulting zone.【Method】This study used polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） technique to analysis the diversity of the V3 region of 16S rRNA genes of bacteria and the change of bacterial genetic composition over time during the sampling period.【Result】Results indicated that the richness（Shannon-Wiener index（H′）） and the abundance（Simpson index（D）） of bacteria population showed significant variation over time during the sampling period,and this change was disordered.When the richness was higher,the abundance was lower.There were no complete overlapped samples in the two-dimensional pattern formed based on the Sorensen similarity coefficient of the samples.【Conclusion】The dynamic change of the bacterial community structure was significant and random in the No.10 sulfur-bearing cold spring over time during the sampling period in Xinjiang faulting zone.

关 键 词：新疆断裂带 含硫冷泉 细菌群落结构动态变化 PCR-DGGE 

分 类 号：S913[农业科学—水产科学]