甲泼尼龙和地塞米松治疗大鼠液压冲击脑损伤的对比研究  

Comparison of methylprednisolone and dexamethasone in treatment of fluid percussion brain injury in rats

作　　者：张兴光[1] 季泰令[1] 王目纲[2] 

机构地区：[1]潍坊医学院附属医院神经外科,山东潍坊261031 [2]河北医科大学第二医院神经外科,河北石家庄050000

出　　处：《成都医学院学报》2011年第1期52-54,共3页Journal of Chengdu Medical College

摘　　要：目的研究甲泼尼龙琥珀酸钠和地塞米松在成年大鼠液压冲击脑损伤中的疗效比较。方法将29只SD大鼠随机分为两组,A创伤组(n=9)和B治疗组(n=20),B组又分为B1甲泼尼龙琥珀酸钠组(n=10)和B2地塞米松组(n=10)。利用液压冲击脑损伤装置建立液压冲击脑损伤模型,A组损伤后不给予特殊处理。B组于损伤1 h、12 h及24 h分别腹腔注射甲泼尼龙琥珀酸钠和地塞米松。伤后72 h干湿重法测定脑组织水含量,机械法制备单细胞悬液并用流式细胞术检测CD11 b平均荧光强度。结果 B组脑组织含水量降低,而且B2高于B1组(P<0.05),各组CD11b表达高低与脑组织水含量趋势一致。结论与地塞米松比较,甲泼尼龙琥珀酸钠更能有效降低脑水肿和小胶质细胞/巨噬细胞的过度激活。Objective To compare the effect of methylprednisolone sodium succinate（MPSS）and dexamethasone in treating fluid percussion brain injury in adult rats.Methods twenty-nine adult sprague-dawley rats were randomly divided into group A（trauma,n=9）and group B（n=20）,which was subdivided into B1（n=10）and B2（n=10）which MPSS or dexamethasone was given 1,12 and 24hr after fluid percussion brain injury.The cerebral injury of all rats was established by the fluid percussion method.Brain water content 72hr after brain injury was determined by the wet and dry weighing method.Single cell suspension was prepared with mechanical method.The florescent intensities for CD11b were analyzed by flow cytometry.Results As far as brain water content and the florescent intensities of CD11b,group A was significantly higher than groups B（P0.05）,and group B2 was higher than group B1（P0.05）.Conclusion MPSS is more effective than dexamethasone in suppressing cerebral edema and over-activation of microglia/macrophages.

关 键 词：地塞米松 甲泼尼龙 脑损伤 

分 类 号：R651.15[医药卫生—外科学]