大肠埃希菌临床菌株生物被膜形成的分析  

Quantitative analysis for biofilm-formation of escherichia coli isolates

作　　者：刘晓峰[1] 迟秀文[2] 黄震[3] 刘丹[1] 潘志雄[1] 

机构地区：[1]九江市第一人民医院检验科,江西九江332000 [2]广东医学院外科护理教研室,广东东莞523808 [3]深圳市龙岗中心医院检验科,广东深圳518116

出　　处：《中华医院感染学杂志》2011年第9期1732-1734,共3页Chinese Journal of Nosocomiology

基　　金：广东医学院青年基金资助(XQ-07010)

摘　　要：目的定量分析大肠埃希菌临床分离株生物被膜的形成。方法采用微量平板培养结合结晶紫染色法建立大肠埃希菌生物被膜定量检测法,并采用该法对49株大肠埃希菌临床菌株生物被膜的形成进行定量分析。结果微量平板培养结合结晶紫染色法可对大肠埃希菌生物被膜进行定量检测。在所检测的49株大肠埃希菌中,有7株菌形成生物被膜的少量,占14.3%;18株菌形成生物被膜的量为中等,占36.7%;24株菌形成生物被膜的量较多,占49.0%。结论绝大多数大肠埃希菌临床菌株能形成中等或较多量的生物被膜。OBJECTIVE To quantitatively analyze the biofilm-formation of Escherichia coliclinical isolates.METHODS The method of microtiterplate culture-crystal violet stain was established for the quantificative analysis of biofilm-formation for the isolates.And 49 E.coliisolates were tested by the method for their biofilm-formation.RESULTS Among 49 E.coliisolates examined,18 isolates formed biofilm of medium quantity,accounting for 36.7%,24 isolates formed more quantity biofilm,accounting for 49.0%,7 isolates formed small quantity of biofilm,accounting for 14.3%.CONCLUSION Most of E.coliclinical isolates can form medium or more-quantity of biofilm.

关 键 词：大肠埃希菌 生物被膜 定量分析 

分 类 号：R378.21[医药卫生—病原生物学]