携带兔斯氏艾美耳球虫MIC-5基因减毒鼠伤寒沙门菌活疫苗的安全性、稳定性与免疫原性  

Safety,stability and immunogenicity of attenuated Salmonella typhimurium carrying MIC-5 gene of Eimeria stieda

作　　者：孟庆玲[1,2] 乔军[1,2] 才学鹏[1] 闫鸿斌[1] 田广孚[1] 骆学农[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]石河子大学动物科技学院,新疆石河子832003

出　　处：《中国兽医学报》2011年第5期659-662,共4页Chinese Journal of Veterinary Science

基　　金：家畜疫病病原生物学国家重点实验室开放课题资助项目(2008001);石河子大学高层次人才专项基金资助项目(RCZX2009001)

摘　　要：对携带兔斯氏艾美耳球虫微线蛋白-5基因(MIC-5)真核表达质粒asd-pBMIC-5-IL-15的减毒鼠伤寒沙门菌X4550(X4550/asd-pBMIC-5-IL-15)进行了安全性、稳定性与免疫原性试验。结果显示,重组菌在109CFU剂量以下对家兔安全;连续培养30代重组菌,重组表达质粒可稳定存在于X4550内,具有良好的遗传稳定性;用重组菌2次免疫动物后,既能诱导机体产生抗兔斯氏艾美耳球虫抗体,也能显著增强淋巴细胞增殖水平,抗球虫指数为164.4。试验表明,减毒沙门菌介导的兔球虫调节型DNA疫苗具有良好的安全性、稳定性和免疫原性。The security,stability and immunogenicity trials of recombinant Salmonella typhimurium X4550 carrying rabbit＇s Eimeria stiedai MIC-5 gene expression plasmid asd-pBMIC-5-IL-15（X4550/asd-pBMIC-5-IL-15） was assayed in this study.The results showed that the 109 CFU inoculation dose was safe in the experimental rabbits.The recombinant plasmid existed stably in X4550 after 30 passages,which displayed a good genetic stability.After two immunizations in rabbits,the recombinant Salmonella typhimurium induced the specific antibodies against rabbit Eimeria stiedai and significantly improved the level of lymphocyte proliferation.The anti-coccidia index reached 164.4.The results indicated that the regulation DNA vaccine mediated by Salmonella had a good security,stability and immunogenicity against rabbit Eimeria stiedai.

关 键 词：斯氏艾美耳球虫 微线蛋白-5基因 减毒鼠伤寒沙门菌 免疫原性 

分 类 号：S852.723[农业科学—基础兽医学]