高钼对雏鸡肝细胞周期和凋亡的影响  

Effect of dietary high molybdenum on the cell cycle and apoptosis of liver in broilers

作　　者：肖杰[1] 崔恒敏[1] 杨帆[1] 彭西[1] 崔伟[1] 程安春[1] 陈涛[1] 柏才敏[1] 

机构地区：[1]四川农业大学动物医学院环境公害与动物疾病四川省高校重点实验室,四川雅安625014

出　　处：《中国兽医学报》2011年第5期734-739,共6页Chinese Journal of Veterinary Science

基　　金："教育部长江学者和创新团队发展计划"创新团队资助项目(IRT0848);四川省重大培育资助项目;四川省科技厅基础应用研究资助项目;四川省教育厅重点资助项目;四川农大"双支"计划创新团队资助项目

摘　　要：300只1日龄健康Avian肉鸡随机分为4组,分别喂以对照组日粮(Mo 13 mg/kg)和高钼日粮(Mo 500 mg/kg,高钼Ⅰ组;Mo 1 000 mg/kg,高钼Ⅱ组;Mo 1 500 mg/kg,高钼Ⅲ组)6周,于14,28,42日龄每天随机抽取5只以流式细胞术研究高钼对雏鸡肝脏细胞周期和凋亡的影响。结果显示:高钼Ⅱ组和Ⅲ组雏鸡体质量和肝脏质量显著低于对照组(P<0.01或P<0.05),脏器指数显著高于对照组(P<0.01或P<0.05)。电镜下肝细胞内质网扩张,线粒体肿胀、空泡化。流式细胞仪测定,高钼Ⅱ组和Ⅲ组肝细胞G0/G1期极显著升高(P<0.01),S期、G2+M期和增殖指数(PI)与对照组相比不同程度地降低;同时,高钼Ⅱ组和Ⅲ组肝细胞凋亡率均极显著高于对照组(P<0.01)。结果表明,日粮含钼1 000 mg/kg及以上时可引起肝细胞分裂增殖能力受阻,凋亡细胞显著增多。Three hundred 1-day-old Avian broilers were divided into four groups and fed on control diet（Mo 13 mg/kg） and high molybdenum diets（Mo 500 mg/kg,groupⅠ;Mo 1 000 mg/kg,groupⅡ;Mo 1 500 mg/kg,group Ⅲ） for 6 weeks.The effect of dietary high molybdenum on the cell cycle and apoptosis of liver in chickens were researched by the methods of flow cytometry（FCM） at 14,28,42 d old.The weight of body and liver was significantly decreased（P0.05 or P0.01）,and the relative weight of liver was significantly increased in group Ⅱand Ⅲ compared with control group（P0.05 or P0.01）.Ultrastructurally,endoplasmic reticulums of hepatocyte were dilated and the mitochondria were swelled or with its crista broken.By FCM,the G0/G1 phase was significantly increased（P0.01）,and S phase,G2＋M phase and PI（proliferating index） significantly decreased（P0.01） in groups Ⅱ and Ⅲ during this experiment.The apoptotic rate was higher in group Ⅱ and Ⅲ than that in control group at 14,28 and 42 days of age（P0.01）.The result showed that dietary molybdenum in excess of 1 000 mg/kg diet could result in the restrained multiplication of hepatocyte and increased apoptosis.

关 键 词：高钼 肝脏 细胞周期 细胞凋亡 流式细胞术 雏鸡 

分 类 号：S856.9[农业科学—临床兽医学]