部分小型猪与大型猪IGF-1基因的SNP分析  

Single nucleotide polymorphism analysis of IGF-1 gene in some miniature pigs and large breeds

作　　者：郎松[1] 邓景致[1,2] 郝林琳[1] 曾养志[3] 王佳[1] 李明谦[1] 程美玲[3] 刘松财[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [3]云南农业大学动物科技学院,云南昆明650201

出　　处：《中国兽医学报》2011年第5期750-753,758,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30740058)

摘　　要：利用PCR-SSCP技术对版纳微型猪、西藏小型猪、军牧猪和大白猪4个品种的IGF-1基因进行了多态性分析。结果表明,在外显子3上存在1个SNP(G201A),在外显子4上存在2个SNPs(A440G和T455C)。各SNP位点基因及基因型频率统计结果显示,在G201A位点,A为大型猪的优势等位基因;在A440G和T455C位点,AT为大型猪的优势等位基因,小型猪的上述2个位点均没有共同的总体特征;2个等位基因型分布差异极为显著(P<0.01),西藏小型猪的多态信息含量最低,而军牧猪的杂合程度最高。PCR-SSCP was applied to analyze the polymorphism of IGF-1 gene in 4 breeds,namely Banna miniature pig,Tibetan miniature pig,＂Junmu-1＂ white pig,large white pig.The results showed that one SNP（G201A） was detected in exon3,two SNPs（A440G and T455C） in exon4.In the G201A,the A allele is an advantage gene in the large breeds;in the A440G and T455C,the AT allele is an advantage gene in the large breeds;no common traits of miniature pig is in the two alleles.The distribution of the two alleles in 4 breeds was significantly different（P0.01）.The polymorphic information content of Tibetan miniature pig is lowest,and the the highest heterozygosity was observed in ＂Junmu-1＂ white pig.

关 键 词：猪 IGF-1基因 PCR-SSCP SNP 

分 类 号：S813.1[农业科学—畜牧学]