影响猪胚胎玻璃化冷冻的若干因素  

Some factors for porcine embryos vitrification

作　　者：张德福[1] 戴建军[1] 吴彩凤[1] 吴华莉[1] 刘东[1] 杨宇[2] 张廷宇[2] 刘伟[3] 殷方芝[3] 王少兵[3] 王昭凯[3] 

机构地区：[1]上海农业科学院畜牧兽医研究所上海市农业遗传育种重点实验室,上海201106 [2]四川农业大学动物科技学院,四川雅安625014 [3]南京农业大学动物科技学院,江苏南京210095

出　　处：《中国兽医学报》2011年第5期754-758,共5页Chinese Journal of Veterinary Science

基　　金：国家转基因专项资助项目(103919N1800);上海市科技兴农重点攻关资助项目[沪农科攻字(2005)第3-5号;(2003)第14-1号];上海市科技兴农重点推广项目[沪农科推字(2007)第3-7号];上海市科学技术委员会成果转化项目(103919N1800);上海市农业遗传育种重点实验室开方课题(Shagb2008-03)

摘　　要：以广西巴马小型猪为供体,采用超数排卵技术,采集5~6日龄的胚胎（囊胚/桑椹胚）,比较2种冷冻方法、胚胎承载工具、透明带处理和冷冻胚胎移植受体对猪胚胎冷冻效果的影响。结果表明,2种冷冻方法的冷冻效果没有显著差异;GMP法能显著提高冷冻胚胎存活率（83.8%vs 77.6%,P〈0.05）和囊胚细胞数（47.5 vs 53.1,P〈0.05）;以0.5%链蛋白酶10 s处理透明带,虽然对猪胚胎存活率没有显著影响,但能显著提高囊胚细胞数（60.1vs 46.6,P〈0.01）;以地方猪种（枫泾母猪）为冷冻胚胎移植受体能显著提高妊娠率和胚胎效率（P〈0.01）。The purpose of this study was to optimize the procedure for cryopreservation of porcine embryos by vitrification.5-6 day-embryos（blastocyst/morula）were collected from superovulated Bama mini-pigs（sows/gilts）.Different cryopreservation methods,cryopreservation tools,thining of zona pellucida（ZP） and recipient breeds were compared.The results were as follows：there were no significant different between two freezing method in embryo survival rate and cell number;the GMP vitrification method was more suitable and efficient in cryopreservation of pig embryos,the embryo survival rate（83.8% vs 77.6%） and blastocyst cell number（53.1 vs 47.5） were significantly higher than the OPS method（P0.05）;there were no significant differences in vitro survival rate by thinning the ZP（0.5% pronase,10 s） after warming but significantly improved the blastocyst cell number in surviving blastcysts（60.1% and 46.6%,P0.01）;the local pig breeds（Fengjing sows） are more suitable as recipients for vitrified/warmed blastcysts in the pregnancy rate and embryos efficiency（P0.01）.

关 键 词：猪 胚胎 玻璃化冷冻 

分 类 号：S814.8[农业科学—畜牧学]