不同化感潜力水稻钾离子吸收动力学差异分析  

Kinetic analysis of potassium absorption in rice accessions with different allelopathic potentials

作　　者：王海斌[1,2] 郭徐魁[2] 周阳[2] 徐志兵[2] 何海斌[1,2] 林文雄[1,2] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [2]福建农林大学农业生态研究所,福州350002

出　　处：《中国生态农业学报》2011年第3期645-649,共5页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金项目(30671220;30471028和31070447);福建省高校服务海西建设重点项目(0b08b005)资助

摘　　要：水培条件下,采用离子耗竭法研究培养液中不同营养元素对化感水稻"PI312777"和非化感水稻"Lemont"钾离子吸收能力的影响,并分析两水稻品种根部钾离子吸收相关基因的差异表达。结果表明,不同处理下,化感水稻"PI312777"相对于对照的△Km、-△Imax和△Cmin变化范围均较小,与非化感水稻"Lemont"相比差异达显著水平。不同处理下,两水稻品种的Km值和Cmin值的变化趋势为完全营养液培养未饥饿处理水稻(KControl)>完全营养液培养饥饿处理水稻(KAll)>只含N、P、K的营养液培养饥饿处理水稻(KNPK)>只含N、K的营养液培养饥饿处理水稻(KNK)>只含P、K的营养液培养饥饿处理水稻(KPK)>无N、P、K的完全营养液培养饥饿处理水稻(KOther)>只含K的营养液培养饥饿处理水稻(KK),最大吸收速率Imax值则相反。可见,不同营养元素对两水稻钾离子吸收存在显著影响,其中N素的影响高于P素,P素高于其他元素。水稻根部钾离子吸收相关基因的差异表达分析结果显示,不同处理条件下两水稻品种钾离子吸收相关基因均上调表达,但非化感水稻"Lemont"的表达强度高于化感水稻"PI312777"。可见,非化感水稻"Lemont"响应环境因素变化的敏感度高于化感水稻"PI312777"。The ion-depletion method was used to explore the differences in two rice accessions（allelopathic rice ＂PI312777＂ and non-allelopathic rice ＂Lemont＂） on potassium ion absorption.Simultaneously,different expressions of the genes involved in potassium ion absorption were determined by FQ-PCR.The results showed that variations in △Km,-△Imax and △Cmin in allelopathic rice ＂PI312777＂ were significantly lower than those in the non-allelopathic rice ＂Lemont＂.The order of Km and Cmin values for two accessions under different treatments was as follow： no starvation treatment with Hoagland solution（KControl） starvation treatment with Hoagland solution（KAll） starvation treatment with nutrient solution containing N,P and K（KNPK） starvation treatment with nutrient solution only containing N and K（KNK） starvation treatment with nutrient solution only containing P and K（KPK） starvation treatment with Hoagland solution without N,P and K（KOther） starvation treatment with nutrient solution only containing K（KK）.The reverse order was observed for Imax.The results showed significant effects of nutrient elements on potassium ion absorption in the two rice accessions.Nitrogen presented the highest effect,which followed by phosphorus.Different gene expressions indicated that the genes related with root potassium ion absorption in the two rice accessions were up-regulated.However,the expressions of the genes in non-allelopathic ＂Lemont＂ were higher than those in allelopathic rice ＂PI312777＂.This suggested that non-allelopathic rice ＂Lemont＂ was more sensitive to environment disturbance than allelopathic rice ＂PI312777＂.

关 键 词：水稻 化感潜力 营养元素 钾离子 吸收动力学 基因表达 

分 类 号：Q945.79[生物学—植物学]