RAW264.7细胞中姜黄素抗动脉粥样硬化作用机制的蛋白质组学研究  

Proteomic research on anti-atherosclerosis mechanism of curcumin in RAW264.7

作　　者：卢德赵[1] 林韬琦[1] 沃兴德[1] 王萍儿[1] 周云[1] 杨贞[1] 

机构地区：[1]浙江中医药大学生命科学学院,浙江杭州310053

出　　处：《中国中药杂志》2011年第9期1207-1211,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30371710)

摘　　要：目的:应用凝胶内差异显示电泳技术和质谱技术研究姜黄素抗动脉粥样硬化的作用机制。方法:RAW264.7细胞和经过25μmol.L-1姜黄素处理的RAW264.7细胞蛋白质经荧光染料Cy3,Cy5标记,与等量Cy2标记的内标混合后在同一胶中进行电泳分离,经不同光激发后扫描得到不同样品的蛋白质组图谱。经DeCyder6.5软件进行差异蛋白质组分析,并对差异蛋白质进行质谱鉴定。结果:ATP合成酶,MHC classⅡ,肌球蛋白轻链,细胞色素b5表达量增加,而磷酸二酯酶4D,elF-3,Hnrpf蛋白,波形蛋白,核仁磷蛋白1,Ran结合蛋白1表达量降低。结论:姜黄素抗动脉粥样硬化作用的机制十分复杂,是其增强细胞抗炎、抗氧化能力以及抑制胆固醇转运,降低细胞内胆固醇积累等因素共同作用的结果。另外,姜黄素可能通过调节细胞的分化及凋亡起到抗肿瘤的作用。Objective：Two-dimensional difference gel electrophoresis and mass spectrum were used to study the anti-atherosclerosis mechanism of curcumin.Method： The proteins from RAW264.7 cell and RAW264.7 cell treated with 25 μmol·L-1curcumin were labeled with Cy3 or Cy5 randomly.Each Cy3-labeled sample and Cy5-labeled sample was mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard and run on the same gel.The gels were scanned under different wavelength light after electrophoresis.All images were analyzed by DeCyder 6.5 software,and the different proteins were identified by mass spectrum.Result： The expression of ATP synthesis H＋ transporting,MHC class Ⅱ,non-muscle myosin alkali light chain and cytochrome b5 increased in the RAW264.7 cell treated with 25 μmol·L-1 curcumin,while the expression of phosphodiesterase 4D,elF-3,Hnrpf protein,vimentin,nucleophosmin1 and Ranbp 1 decreased.Conclusion： The anti-atherosclerosis mechanism of curcumin is the result of enhancement of the cell inflammation,antioxidant activity and inhibition of cholesterol transport,reduce of the accumulation of intracellular cholesterol and other factors.In addition,curcumin also had the effect of anti-tumor through regulated tumor cell differentiation and apoptosis.

关 键 词：姜黄素 动脉粥样硬化 蛋白质组 RAW264.7 

分 类 号：R285[医药卫生—中药学]