基于生物标志物变化的黄芩干预肺热证候研究  

Researches on pyretic pulmonary syndrome model interfered by Scutellariae Radix based on variation of biomarker

作　　者：刘树民[1] 柳长凤[1] 祖金祥[1] 李俊行[1] 汪娜[1] 卢芳[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2011年第9期1212-1216,共5页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2007CB512608);哈尔滨市科技创新人才研究专项项目(2008RFXXS042)

摘　　要：目的:通过黄芩干预肺热证候的代谢组学研究,确定表征肺热证候的特异性生物标志物群,并基于这些生物标志物的变化探讨黄芩清肺热的作用机制。方法:基于代谢组学理论,以UPLC-Q-TOF/MS为核心技术,运用主成分分析法(PCA)为数据解析手段,研究空白大鼠、肺炎链球菌诱导肺热证候大鼠和黄芩干预大鼠尿液中代谢物组时量的轨迹变化和对应关系。结果:确定6个能够表征肺热证候的特异性生物标志物,且黄芩对6个特异性生物标志物具有明显的回调作用,初步鉴定了2个生物标志物:D-12-前列腺素J2和吲哚酚硫酸盐。结论:从生物体内代谢层面阐明黄芩具有较好的清肺热作用,并且清肺热作用机制与6个生物标志物的代谢相关联。Objective：Metabonomics researches of Scutellariae Radix interfering pyretic pulmonary syndrome had been done,to determine the specific biomarkers of pyretic pulmonary syndrome,and to approach the mechanism that Scutellariae Radix interfered the variation of these biomarkers.Method： Metabonomics technique,UPLC-Q-TOF/MS analytical means and PCA statistical methods were utilized to investigate the trajectory change and inter-relationship of urinary metabolome of rats treated differently.Result： Six specific biomarkers were determined which could represent Streptococcus pneumoniae-induced pyretic pulmonary syndrome in rats.Scutellariae Radix could significantly adjust the ascended biomarkers to the normal level.Meanwhile two of these biomarkers were identified as Delta-12-prostaglandin J2 and indoxyl sulfate.Conclusion： There was a good therapeutic function of Scutellariae Radix on pyretic pulmonary syndrome,which was elucidated on the metabolic aspects.There was also a correlationship between the mechanism of Scutellariae Radix curing pyretic pulmonary syndrome and the six specific biomarkers.

关 键 词：生物标志物 黄芩 肺热证候 代谢组学 UPLC-Q-TOF/MS 

分 类 号：R285[医药卫生—中药学]