蜘蛛香环烯醚萜对肠易激综合征模型大鼠5-HT和5-HIAA的影响  

Influence of iridoid from Valeriana jatamansi on 5-HT and 5-HIAA in rats with irritable bowel syndrome

作　　者：闫兴丽[1] 洪缨[1] 石晋丽[1] 秦谊[1] 张建军[1] 林清[1] 陈振振[1] 赵仁 崔小丽 高学敏[1] 

机构地区：[1]北京中医药大学,北京100029 [2]云南省药物研究所,云南昆明650111 [3]河北省承德市药品检验所,河北承德067000

出　　处：《中国中药杂志》2011年第9期1235-1238,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572463;81073132);校级自主课题(2009JYBZZ-JS064)

摘　　要：目的:探讨蜘蛛香环烯醚萜治疗肠易激综合征(IBS)的作用机制。方法:雄性SD大鼠60只,分为空白组、模型组、氟西汀组和蜘蛛香环烯醚萜高、中、低剂量组(24.92,12.46,6.23 mg.kg-1),各10只,以慢性应激加孤养的方法造模,观察蜘蛛香环烯醚萜对各组结肠、血清和下丘脑中5-HT和5-HIAA含量的影响。结果:模型组结肠、血清含量均显著上升,而下丘脑5-HT含量显著下降,5-HIAA和5-HT/5-HIAA变化则不太明显,蜘蛛香环烯醚萜各剂量组对结肠和血清中的5-HT有明显的降低作用,对下丘脑中5-HT则有升高作用。对5-HIAA的影响则无明显变化规律,而对结肠和血清中5-HT/5-HIAA则表现为降低作用。结论:蜘蛛香环烯醚萜改善IBS症状的机制可能与调节从胃肠到中枢的5-HT水平有关。Objective：To investigate the mechanism of iridoid from Valeriana jatamansi treating irritable bowel syndrome.Method： Sixty male SD rats were equally divided into 6 groups（2 controls,1 model and 3 treatment doses） with 10 rats per group.The test groups were administered with iridoid（24.92,12.46,6.23 mg·kg-1） while the control groups were administered with fluoxetine（2.5 mg·kg-1,positive control） or distilled water（negative control）.The model was established by chronic stress and independent feeding.The influence of iridoid from V.jatamansi on 5-HT and 5-HIAA in colon,serum and hypothalamic were observed in all groups.Result： In the model group,the content of 5-HT in colon and serum increased significantly,but the content of 5-HT in hypothalamic decreased significantly.The content of 5-HIAA and the value of 5-HT/5-HIAA had no significant change.In three iridoid-treated groups,the content of 5-HT in colon and serum decreased,but the content of 5-HT in hypothalamic increased.The content of 5-HIAA had no significant change.The value of 5-HT/5-HIAA in colon and serum reduced.Conclusion： The mechanism of iridoid from V.jatamansi treating irritable bowel syndrome may be related to the regulation effect to the levels of 5-HT from Gastrointestinal to central nervous system.

关 键 词：蜘蛛香 环烯醚萜 肠易激综合征 5-HT 5-HIAA 

分 类 号：R285.5[医药卫生—中药学]