缺氧缺血性脑损伤新生大鼠脑室管膜下区Foxg1与p21基因的表达及其关系  

Expression and Relationship between Foxg1 Gene and p21 Gene in Sub Ventricular Zone in Cerebral Tissue of Newborn Rats with Hypoxic-Ischemic Brain Damage

作　　者：王军[1] 侯艳艳[1] 朱登纳[1] 吴值荣[1] 范亚珍[1] 陈海[1] 熊华春[1] 王留霞[1] 

机构地区：[1]郑州大学第三附属医院脑瘫康复科

出　　处：《实用儿科临床杂志》2011年第8期606-608,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省医学科技攻关项目(2007030066)

摘　　要：目的观察缺氧缺血性脑损伤(HIBD)新生大鼠脑室管膜下区Foxg1基因与p21基因表达的动态变化,探讨二者的相互关系。方法采用R ice法制作新生大鼠HIBD动物模型,假手术组作为对照,分别在术后第3、7、14、21、28天处死,取其左侧大脑半球,应用原位杂交法测定HIBD模型组与对照组新生大鼠Foxg1基因与p21基因在脑室管膜下区(SVZ)不同时间点表达水平。结果 1.HIBD后SVZ区Foxg1基因3 d时与假手术组比较已明显升高(P<0.05),7 d时表达达高峰,此后逐渐下降,各时间点表达水平均较假手术组高(Pa<0.05),假手术组各时间点Foxg1表达水平变化不大。2.HIBD后p21基因3 d时表达较假手术组明显升高(P<0.05),7 d时表达水平最低,此后又逐渐升高,各时间点表达水平均较假手术组高(Pa<0.05),假手术组也有升高趋势。3.二组p21基因与Foxg1基因均呈负相关。结论 HIBD可以促进新生大鼠脑组织SVZ区Foxg1基因表达,HIBD后SVZ区细胞凋亡增加,p21基因表达上调。Foxg1基因可能是p21基因的上游调节基因。Objective To observe the alteration in Foxg1 gene,p21 gene levels in the sub ventricular zone（SVZ） in cerebral tissues of newborn rats with hypoxic-ischemic brain damage（HIBD）,and to explore the relationship between the two genes.Methods The HIBD models were induced by Rice method.The control group was induced without carotid artery ligation.The expressions of each gene in the SVZ were examined by in-situ hybridization on the 3rd,7th,14th,21st,28th day in two groups.Results 1.Compared with the control group,the expression of Foxg1 in HIBD group was higher on the 3rd day,highest on the 7th day,and then went down slowly in the 14th,21st,28th day.The total expression level was higher than that in the control group（P0.05）;and the level changes was not so outstanding in the control group.2.Compared with the control group,p21 was much higher on the 3rd group,lower on the 7th day,then became to increase on the 14th,21st,28th day,the total expression level trend was higher than that in the control group（P0.05）,and it was also in a upward trend.3.The correlations between Foxg1 and p21 were negative in two groups.Conclusions HIBD contributes to the expression of Foxg1 genes in the SVZ in cerebral tissues of newborn rats,HIBD leads to cell apoptosis which promotes the expression of p21 gene.Foxg1 gene may be the upstream gene of p21.

关 键 词：缺血缺氧性脑损伤 Foxg1基因 P21基因 

分 类 号：R722.1[医药卫生—儿科]