玉米非编码RNA Zm-1701在逆境胁迫下的表达研究  

Expression analysis of maize non-coding RNA Zm-1701 in response to drought and salt stress

作　　者：刘铁梅[1] 刘宗才[2] 李丹丹[2] 高美娟[1] 张琨[1] 阚云超[2] 李春奇[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]南阳师范学院河南省伏牛山昆虫生物学重点实验室,河南南阳473061

出　　处：《河南农业大学学报》2011年第2期137-141,共5页Journal of Henan Agricultural University

基　　金：国家重点基础研究发展计划(973计划)子项目(2009CB825400)

摘　　要：分别用20%PEG6000,150 mmol·L-1NaCl,100μmol·L-1ABA对3叶期的玉米根进行胁迫处理,通过分析3种胁迫应答基因的表达情况确定胁迫的有效时间点,进一步研究玉米非编码RNAZm-1701在玉米受到干旱和盐胁迫处理时的表达情况.结果表明,玉米根在受到干旱和盐胁迫后,根中非编码RNAZm-1701的表达量都有上升的趋势,且Zm-1701对盐胁迫的反应灵敏程度高于干旱胁迫.表明该ncRNA可能参与到玉米的胁迫早期应答反应过程中.To analyze the regulatory mechanism of non-coding RNAs （ncRNAs） in root growth, three simulated stress conditions with 20% PEG6000, 150 mmol - L-1 NaC1, 100 ixmol - L-1 ABA were implemented to maize root at trefoil stage. Based on the expression of three stress response genes, we chose the proper time points of drought and salt treatments, and further studied the expression profile of non-coding RNA Zm-1701. Results showed that the expressions of Zm-1701 all increased after PEG6000, NaC1 and ABA treatments. Moreover, the reaction of Zm-1701 to salt stress was more sensitive than that to drought. These findings indicated that Zm-1701 might participate in early responses in maize resistance to salt and drought stress.

关 键 词：玉米 胁迫 非编码RNA 

分 类 号：S513[农业科学—作物学]