用流式细胞术分析双氢青蒿素对杜氏利什曼原虫DNA的作用  被引量:3

EFFECT OF DIHYDROARTEMISININ ON LEISHMANIA DONOVANI PROMASTIGOTES DNA ANALYSED BY FLOW CYTOMETRY

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作  者:芦殿梅[1] 胡孝素[1] 乔中东[2] 

机构地区:[1]华西医科大学寄生虫学研究室,成都6100412 [2]山西医科大学分子生物学研究室,太原030001

出  处:《实用寄生虫病杂志》1999年第3期97-99,共3页Journal of Practical Parasitic Diseases

基  金:国家教 委博士学科点 专项基 金

摘  要:应用流式细胞术( F C M )检测分析了双氢青蒿素对杜氏利什曼原虫前鞭毛体 D N A 的作用。4 个不同浓度(177×10- 4,353×10- 4,71×10- 4,141×104m ol/ L)的药物对虫体 D N A 均有抑制作用,用药组虫体 D N A 含量的荧光分布随着药物浓度的升高而减弱,抑制率分别为 323% ,326% ,864% ,892% ,与对照组相比有显著性差异( P< 005)。后两个用药组与前两个用药组抑制率有显著性差异( P< 0005)。本研究结果与光镜观察、3 H Td RIn the paper, the effect of dihydroartemisinin on Leishmania donovani promastigotes DNA is analysed by flow cytometry. L.donovani promastigotes DNA was inhibited by dihydroartemisinin under four different concentrations (1.77×10 -4 , 3.53×10 -4 , 7.1×10 -4 , 14.1×10 -4 mol/L). In experiment groups, the fluorescent distribution of DNA content in L.donovani promastigotes decreased when drug concentration increased and with inhibition rates of 32.3%, 32.6%, 86.4% and 89.2% respectively. There were significant differences between the former two concentration groups and the latter two concentration groups ( P <0.005). Besides, there were also significant differences between 4 experiment groups and control group ( P <0.05). The result was coincident with that of microscopy observation and 3 H TdR marking experiment.

关 键 词:双氢青蒿素 杜氏利什曼原虫 DNA 流式细胞术 

分 类 号:R382.22[医药卫生—医学寄生虫学]

 

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