Toll样受体在不同转移能力人乳腺癌细胞株中的差异表达及意义  

Differential expression and its significance of Toll like receptors in human breast cancer cells with different invasive capacities

作　　者：周晓妮[1] 杨欢[1] 温会燕[1] 周惠琴[1] 冯萍[1] 朱雪明[1] 

机构地区：[1]苏州大学附属第二医院检验科,215004

出　　处：《中华实验外科杂志》2011年第5期662-664,共3页Chinese Journal of Experimental Surgery

基　　金：苏州市科技计划资助项目（SYS201032）

摘　　要：目的观察人高低转移能力乳腺癌细胞株中Toll样受体（TLRs）的表达，探讨差异表达及意义。方法选取高转移能力的人乳腺癌细胞株MDA—MB231和低转移能力的人乳腺癌细胞株MCF-7，采用逆转录-聚合酶链反应（RT—PeR）、实时荧光定量PCR、流式细胞技术和细胞免疫荧光技术检测TLR1～TLR10mRNA水平和蛋白水平的表达，观察两株细胞TLRs的表达及表达差异。结果人乳腺癌细胞株MDA．MB-231和MCF-7mRNA水平和蛋白水平都广泛表达TLRs包括TLR1、TLR2、TLR3、TLR4、TLR5、TLR6、TLR7、TLR8、TLR9、TLRIO。两细胞株mRNA水平表达差异最明显的是TLR4、TLR6、TLR8和TLR9，MDA—MB-231中TLR4、TLR6、TLR8和TLR9的表达分别是MCF-7的（39．4±3，2）倍、（10．8±2．4）倍、（5．8±2．6）倍和（3．2±2．5）倍（P〈0．01）；两细胞株蛋白表达差异最大的是TLR4、TLR6、TLR7和TLR5，MDA—MB-231中TLR4、TLR6、TLR7和TLR5的表达分别是MCF-7的（6．4±1．6）倍、（4．1±0．9）倍、（3．7±0．8）倍和（2．8±0．4）倍（P〈0．01）。结论TLRs在人高低转移能力乳腺癌细胞株广泛表达且表达水平不-；TLR4是表达差异最明显的受体；差异表达的TLRs可能与乳腺癌细胞转移能力相关。Objective To study the expression and significances of Toll like receptors （TLRs） in human high invasive breast cancer cells MDA-MB-231 and poor invasive breast cancer cells MCF-7. Methods TLRs mRNA expression levels were detected in breast cancer cell lines MDA-MB-231 and MCF-7 by reverse transcription-polymerase chain reaction （RT-PCR） and real-time PCR. TLRs protein expression levels were detected by flow cytometry （FCM） and immunofluorescence methods. Results Human breast cancer cell lines MDA-MB-231 and MCF-7 expressed TLRs including TLR1-TLR10 at both mRNA and protein levels. The mRNA expression of TLR4, TLR6, TLR8 and TLR9 in MDA-MB-231 cells was obviously higher than that in MCF-7 cells respectively, and the expression in MDA-MB-231 cells was （39.4 ± 3.2）, （ 10. 8±2.4）, （5.8 ±2.6） and （3.2 ± 2.5 ） times as much as that in MCF-7 cells respectively （P 〈 0. 01 ）. Protein expression of TLR4, TLR6, TLR7 and TLR5 in MDA-MB-231 cells was significantly higher than that in MCF-7 cells respectively, and the expression in MDA-MB-231 cells was （6.4 ± 1.6）, （4. 1 ± 0. 9）, （ 3.7 ± 0. 8 ） and （ 2. 8 ± 0. 4 ） times as much as that in MCF-7 cells respectively （ P 〈 O. 01）. Conclusion TLR1-TLR10 was expressed widely and differentially in human breast cancer cell lines MDA-MB-231 and MCF-7. TLR4 mRNA and protein levels were the highest out of TLR1-TLRIO. The differential expressions of TLRs may be related to the invasion capacity of human breast cancer cells. TLR4 may provide basement for breast cancer target gene therapy.

关 键 词：TOLL样受体 乳腺癌 转移 

分 类 号：R737.9[医药卫生—肿瘤]