SCF／c—kit基因在隐睾大鼠睾丸中的表达及其意义  

Change and significance of the expression of SCF and c-kit in the experimental cryptorchidism rat testis

作　　者：徐明[1] 郑新民[2] 丁协刚[2] 周克文[2] 李世文[2] 汪聪[2] 

机构地区：[1]武汉大学中南医院胸外科,430071 [2]武汉大学中南医院泌尿外科,430071

出　　处：《中华实验外科杂志》2011年第5期781-783,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察隐睾大鼠睾丸组织中细胞凋亡及SCF／c—kit基因及其表达产物的变化，探讨隐睾致生精细胞受损的机制。方法30只SD大鼠随机分为对照组和实验组，实验组复制单侧（左侧）腹腔隐睾模型。1个月后分别取两组左侧睾丸组织进行real—time逆转录-聚合酶链反应（RT—PCR）、Westernblot及免疫组织化学检测SCF／c—kit基因及其蛋白表达变化，TUNEL法检测细胞凋亡。结果与对照组比较实验组隐睾生精细胞凋亡明显增加，对照组与实验组凋亡指数分别为5．4±1．02、19．7±3．83，差异有统计学意义（P〈0．05）；实验组SCF、C—kitmRNA的表达相对含量分别为0．57±0．04、0．38±0．06，差异有统计学意义（P〈0．05）；SCF和c—kit蛋白在对照组和实验组相对表达含量分别为1．52±0．47、0．61±0．21；0．89±0．13、0．38±0．07，差异有统计学意义（P〈0．05）；SCF与c—kit均有显著相关（r=0．955，P〈0．01）。结论隐睾可致睾丸生精上皮细胞凋亡增加且与SCWc—kit基因表达异常有关。Objective To investigate the germcell apoptosis and the change in expression levels of SCF and c-kit in the experimental cryptorchidism rat testis. Methods 30 male SD rats were divided into two groups：control group and experimental group. The control group was treated with sham-operation on the left side testis and the experimental cryptorchidism group was treated on the same side by surgery of displacing the testis inside the abdominal cavity ,followed by suture of the inguinal canal. After 1 months the rats were sacrificed and the testes were harvested. The histopathological changes were observed by microscope and the expression of SCF and c-kit were detected by immunohistochemical assay, quantitative Real-time reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting analysis. Cell apoptosis was detected by TUNEL. Results The testis weight of unilateral cryptorichid rats reduced greatly and marked germ cell loss could be seen when compared to the control group and the incidence of apoptosis in control group and experimental cryptorchidism group was 5.4 ± 1.02,19.7 ±3.83 respectively（P 〈0. 05）. The c-kit and SCF mRNA expression was 0. 38 ±0. 06,0. 57 ± 0. 04 respectively, decrease dramatically when compared to the control group （ P 〈 0. 05 ）. The protein expression of SCF and c-kit were detected in both control group and experimental cryptorchidism group with 1.52± 0. 47,0. 61 ± 0. 21,0. 89 ± 0. 13,0. 38 ± 0. 07 （ P 〈 0. 05 ）. Conclusion The decreased expression of SCF and c-kit may play a very important role in germ cell apoptosis caused by experimental cryptorchidism.

关 键 词：隐睾 干细胞生长因子 受体 

分 类 号：R698.2[医药卫生—泌尿科学]