一种适用于长期定量观察生物活细胞的数字全息显微方法  

Digital Holographic Microscopy for Long-Term Quantitative Phase-Contrast Imaging of Living Cells

作　　者：潘锋[1] 肖文[1] 刘烁[1] 

机构地区：[1]北京航空航天大学仪器科学与光电工程学院,北京100191

出　　处：《中国激光》2011年第5期225-228,共4页Chinese Journal of Lasers

基　　金：国家自然科学基金(31000387);中央高校基本科研业务费专项资金(YWF-10-02-087)资助课题

摘　　要：动态定量观察生物样品对生命与生物科学研究具有重要意义。研究了一种适用于长期定量观察生物活细胞的数字全息相衬显微成像方法。针对实际生物细胞显微观察中通常生物样品处于半封闭状态,设计了一种光纤与空间光混合的全息记录光路。针对长期观察过程中光学系统和细胞培养基引起的相位畸变,通过在全息面和成像面分别进行像差校正,获得高质量的样品相衬图像。并对造骨细胞MC3T3-E1和骨细胞MLO-Y4进行了长时间的显微观察实验,验证了该方法。Dynamic and quantiative observing on the biological samples is important for life and biological sciences research. A digital holographic microscopy for long-term quantitative phase-contrast imaging of living cells is proposed. At the condition that biological cells for microscopic observation are usually in semi-enclosed environment actually, the optical configuration of digital holographic microscopy is designed and optimized by using free-space-fiber hybrid optical structure. Considering the additional phase aberration induced by liquid culture medium in long-term observation, the system aberrations are numerically corrected at the hologram plan and image plan, in order to obtain high quality phase contrast images. The living samples of MC3T3-E1 and MLO-Y4 cells are investigated by long-term microscopic observation, and the experimental results are given to confirm the Droposed method.

关 键 词：全息 相衬成像 显微观察 生物细胞 

分 类 号：TN247[电子电信—物理电子学] O438[机械工程—光学工程]