ApoB基因多态性与HCV感染易感性关系  

Relationship between the polymorphism of apoliprotein B gene and hepatitis virus C infection

作　　者：刘燕[1] 杨静静[2] 喻荣斌[1] 王昊鹏[1] 王洁[1] 崔倩[1] 邓小昭[3] 张云[1] 

机构地区：[1]南京医科大学公共卫生学院流行病与卫生统计系,江苏南京210029 [2]河海大学校医院 [3]南京军区军事医学研究所

出　　处：《中国公共卫生》2011年第5期558-560,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81072343)

摘　　要：目的探讨载脂蛋白B(ApoB)基因单核苷酸多态性C-2488T和G-4154A在病例组与对照组的分布及与HCV感染的遗传易感性关联。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法,对190例HCV感染者和398例对照组人群进行C-2488T和G-4154A多态性检测,比较不同基因型与HCV感染易感性关系。结果 G-4154A位点病例组和对照组基因型为GG、GA、AA分别有160、27、3例和364、32、2例,基因型频率分别为84.2%、4.2%、1.6%和91.5%、8.0%、0.5%,差异有统计学意义(P<0.05);病例组和对照组等位基因为A、G分别有347、33例和760、34例,差异有统计学意义(P<0.05);C-2488T位点病例组和对照组基因型为CC、CT、TT分别有176、14、0例和357、40、1例,基因型频率分别为92.6%、7.4%、0.0%和89.7%、10.1%、0.3%,差异无统计学意义。结论江苏省汉族人群ApoB基因多态性改变可能与HCV感染的遗传易感性有关。Objective To investigate the relationship between two functional polymorphisms of the ApoB gene and infection of hepatitis C virus（HCV）.Methods PCR-restriction fragment length polymorphism（PCR-RFLP） technique was used to analyze the genotype of the ApoB gene in 190 anti-HCV positive hemodialysis patients（cases） and 398 anti-HCV negative hemodialysis patients frequency-matched to the cases by age and sex.Results The genotype frequency of GG,GA,and AA of G-4154A was 84.2%,4.2%,and 1.6% for the cases and 91.5%,8.0%,and 0.5% for the controls with significant differences between the two groups（P0.05 for all）.The number of potients with A allele and G allele were 347 and 33 for the cases and 760 and 34 for the controls with significant difference between the two groups.There was no significant difference in genotype frequency of C-2488T polymorphism between cases and controls.Conclusion The results reveal that the polymorphism of the ApoB gene may be associated with the susceptibility to HCV infection.

关 键 词：丙型肝炎病毒 载脂蛋白B基因 病例对照研究 多态性 

分 类 号：R181[医药卫生—流行病学]