苯并[a]芘对大鼠学习记忆及海马神经元影响  

Morphology in hippocampal neurons and behavioral change in rats with sub-chronic exposure of benzo(a) pyrene

作　　者：陈承志[1] 汤艳[2] 蒋学君[3] 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院劳动卫生与环境卫生教研室,重庆400016 [2]泸州医学院公共卫生系劳动卫生与环境卫生教研室 [3]四川大学华西公共卫生学院环境卫生教研室

出　　处：《中国公共卫生》2011年第5期608-610,共3页Chinese Journal of Public Health

基　　金：国家自然科学资金(30671744)

摘　　要：目的探讨苯并[a]芘(BaP)对大鼠学习记忆及海马神经元的影响。方法将50只SD大鼠随机分为空白对照组、溶剂对照组、BaP低(2.5 mg/kg)、中(5 mg/kg)、高(10 mg/kg)剂量组,腹腔注射染毒,每天1次,持续30d;用Morris水迷宫测试学习记忆,电镜观察海马神经元超微结构。结果空白对照组、溶剂对照组和BaP低、中、高剂量组穿越平台次数分别为(12.03±2.43)、(11.35±2.68)、(9.75±1.78)、(7.63±1.67)、(4.38±1.46)次,在平台象限停留时间分别为(46.34±11.56)、(48.12±13.22)、(32.35±7.68)、(26.13±5.34)、(16.14±3.15)s,差异均有统计学意义(P<0.05);随染毒剂量的增加,BaP低、中、高剂量组大鼠的平均逃避潜伏期和第1次寻找平台的潜伏期均延长,平均有效策略百分比、穿越平台的次数均减少,在平台象限停留时间均缩短,差异均有统计学意义(P<0.05);电镜观察结果显示,BaP低剂量组海马神经元胞体浓缩变性,核膜皱缩变形;中剂量组出现线粒体肿胀;高剂量组出现高尔基扩张。结论 BaP亚慢性染毒对大鼠具有一定的神经行为毒性。Objective To explore the effects of benzo（a）pyrene（BaP） exposure on spatial learning and memory abilities and ultramicrostructure of hippocampal neurons in rats.Methods Fifty healthy male SD rats were randomly divided into a control group,a solvent control group and three BaP-treated groups.The exposure groups were treated by intraperitoneal injection with 2.5,5,and 10 mg/kg BaP once a day,respectively.The rats in solvent control group received an equal volume of corn oil.The treatment was lasted for 30 days.The capability of learning and memory of the rats were measured by Morris water maze test.The specimens of hippocampus were sliced and the morphological changes were observed with transmission electron microscopy.Results The times of passing through the platform were 12.03±2.43,11.35±2.68,9.75±1.78,7.63±1.67,and 4.38±1.46 and the time（in second） of staying around the platform were 46.34±11.56,48.12±13.22,32.35±7.68,26.13±5.34,and 16.14±3.15,respectively,for the rats of blank control,solvent control,low-,moderate,and high-dose BaP treatment group.A significant impairment in Morris water maze performance among the BaP-treated rats was observed compared with the controls（P0.05）.The results also showed that damages of ultramicrostructure of hippocampal neurons were significant in the BaP-treated groups compared with that of the controls.Conclusion BaP has neurobehavioral toxicity in rats.

关 键 词：苯并[A]芘 学习记忆能力 MORRIS水迷宫 海马 

分 类 号：R136.32[医药卫生—劳动卫生]