菘蓝rDNA及端粒多色荧光原位杂交分析  

Multicolor fluorescence in situ hybridization analysis on rDNA and telomere of Isatis indigotica

作　　者：杨飞[1] 李立家[2] 

机构地区：[1]长江大学医学院分子生物学部,湖北荆州434023 [2]武汉大学生命科学学院,植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《中草药》2011年第5期972-975,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30821064);湖北省教育厅科学研究项目(Q20111307)

摘　　要：目的对菘蓝45S rDNA、5S rDNA和端粒进行物理定位,并分析其核型。方法使用多色荧光原位杂交技术(FISH)进行定位。结果菘蓝基因组有一对45S rDNA位点位于4号染色体,一对5S rDNA位点位于5号染色体,14对端粒信号位于染色体两端;核型公式为2n=2x=14=10m(2SAT)+4sm,属2A核型。结论为菘蓝核型分析、分子细胞遗传图谱构建提供了稳定、有效的细胞学标记。Objective To investigate the physical location of 45S,5S rDNA,and telomere sequences and analyze the karyotype of the metaphase chromosomes of Isatis indigotica.Methods Multicolor fluorescence in situ hybridization（FISH） approach was used to detect the physical location of the above sequences.Results One pair of 45S rDNA sites located on chromosome 4,one pair of 5S rDNA sites located on chromosome 5 and 14 pairs of telomere sites located at the ends of each chromosome in I.indigotica were detected.The karyotype formula is 2n = 2x = 14 = 10m（2SAT） ＋ 4sm,which belongs to 2A karyotype.Conclusion This research provides effective cytological markers for karyotype analysis and construction of a molecular cytogenetic map of I.indigotica.

关 键 词：菘蓝 RDNA 端粒 荧光原位杂交 核型 

分 类 号：R282.12[医药卫生—中药学]