无花果叶补骨脂素对体外培养瘢痕疙瘩成纤维细胞的影响  

Influence of Psoralen of Fig Leaf on Human Keloid Fibroblasts in Vitro

作　　者：王涛[1] 陶如[2] 李清华[3] 高永峰[4] 周成军[5] 刘金成[6] 

机构地区：[1]泰山医学院基础医学部生物化学教研室,泰安市271000 [2]泰山医学院临床医学部,泰安市271000 [3]泰山医学院附属医院,泰安市271000 [4]泰山医学院药学院药学综合实验室,泰安市271000 [5]山东大学第二医院,济南市250033 [6]青岛大学医学院生物学教研室,青岛市266021

出　　处：《中国药房》2011年第19期1746-1749,共4页China Pharmacy

基　　金：山东省中医药管理局资助项目(2007167);泰山医学院青年基金资助项目(997)

摘　　要：目的:研究无花果叶补骨脂素(FLP)对瘢痕疙瘩成纤维细胞的生长抑制作用。方法:用FLP处理体外培养的成纤维细胞后进行细胞形态学检测,通过细胞增殖试验(MTT法)和流式细胞仪检测FLP对成纤维细胞增殖和凋亡的影响;氯胺T法检测FLP对成纤维细胞胶原蛋白合成的抑制作用;Western-bloting检测凋亡相关蛋白Bcl-2的表达。结果:经FLP处理后,成纤维细胞增殖活性降低,凋亡指数升高(P<0.01),凋亡相关蛋白Bcl-2表达被抑制,同时胶原蛋白的合成受到抑制。结论:FLP对瘢痕疙瘩成纤维细胞增殖的抑制和诱导其凋亡作用可能是通过抑制凋亡相关蛋白Bcl-2的作用来实现的。OBJECTIVE：To investigate the effect of psoralen of fig leaf（FLP） on the proliferation and apoptosis of human keloid fibroblasts. METHODS：Human keloid fibroblasts were treated with FLP. Morphology,MTT method and flow cytometry（FCM） were adopted to detect the effect of FLP on the proliferation and apoptosis of human keloid fibroblasts. The inhibition effect of PSO on the synthesis of fibroblasts collagen was determined by chloramines T assay. The protein levels of Bcl-2 were detected by Western bloting. RESULTS：After FLP treatment,the proliferation activity of human keloid fibroblasts decreased while the apoptotic index increased（P0.01） . Related-apoptosis Bcl-2 protein level was inhibited as well as the synthesis of college protein. CONCLUSION：FLP inhibits the proliferation and induces the apoptosis of human keloid fibrolasts by means of inhibiting related-apoptosis Bcl-2.

关 键 词：无花果叶 补骨脂素 瘢痕疙瘩 成纤维细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]