毛细管电泳法测定白芥子中芥子碱的含量  

Determination of the content of sinapine in seeds of Sinapis alba L. by capillary electrophoresis

作　　者：苑艳霞[1] 王惠国[1] 刘燕妮[2] 尤慧艳[3] 冯宝民[1] 

机构地区：[1]大连大学生物工程学院,辽宁大连116622 [2]大连大学信息工程学院,辽宁大连116622 [3]大连大学环境与化学工程学院,辽宁大连116622

出　　处：《沈阳药科大学学报》2011年第5期384-386,395,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(30600802);辽宁省高等学校优秀人才支持计划资助项目(2008RC03)

摘　　要：目的建立毛细管电泳法测定白芥子中芥子碱含量的方法。方法采用未涂层熔融石英毛细管柱（52 cm×100μm ID,有效分离长度42 cm）为分离通道,运行缓冲液为15 mmol.L-1柠檬酸-15mmol.L-1柠檬酸三钠（pH=5.0）,操作电压15 kV（恒压）,检测波长254 nm,毛细管柱温为25℃,电动进样7 kV×3 s。结果芥子碱硫氰酸盐质量浓度在0.5~20 mg.L-1内线性关系良好（r=0.998 9）,平均加样回收率为97.8%（RSD值为2.36%,n=5）。白芥子中芥子碱的平均含量为4.23 mg.g-1,分析时间为5 min。结论用毛细管电泳法测定白芥子中芥子碱含量的方法可行、操作简单、检测快速。Objective To develop the capillary electrophoresis method for quantitative analysis of sinapine in the seeds of Sinapis alba L.Methods A fused silica capillary（52 cm×100 μm ID,an effective length of 42 cm）was used.The running buffer was composed of 15 mmol·L-1 citric acid-15 mmol·L-1 sodium citrate（pH=5.0）.The applied voltage was 15 kV and the capillary temperature was 25 ℃.The detection wavelength was set at 254 nm.The injection condition was 7 kV× 3 s.Results The content of sinapine in the seeds was 4.23 mg·g-1.The analysis time was 5 min.A good linearity was obtained in the range of 0.5-20 mg·L-1（r=998 9）for sinapine.The average recoveries and RSD were 97.3% and 2.72%（n=5）,respectively.Conclusions The method is simple and rapid and it has satisfactory recoveries and good reproducibilities.

关 键 词：白芥 种子 芥子碱 毛细管电泳法 含量测定 

分 类 号：R917[医药卫生—药物分析学]