光合细菌菌剂在提高板蓝根品质中的应用研究  

Application of Photosynthetic Bacteria in improving the quality of Radix Isatidis

作　　者：李军[1] 张辉[1] 李建文[1] 白红娟[2] 杨官娥[1] 

机构地区：[1]山西医科大学药学院,山西太原030001 [2]中北大学化工与环境学院,山西太原030051

出　　处：《中国医药导报》2011年第13期58-59,共2页China Medical Herald

基　　金：山西省科技攻关项目(项目编号:051081);山西医科大学大学生创新项目

摘　　要：目的:考察在板蓝根种植中应用光合细菌菌剂后板蓝根和大青叶药材品质的提高。方法:采用高效液相色谱法,以甲醇-水(75:25)为流动相,检测波长为289 nm,分析大青叶中靛玉红的含量;按药典要求,用45%乙醇做溶剂测定板蓝根中醇溶性浸出物的含量。结果:使用光合细菌菌剂后,大青叶中靛玉红含量由0.11 mg/g增加为0.26 mg/g,增加了136.4%;板蓝根中浸出物的含量由42.2%增加为47.2%,提高了11.8%。结论:光合细菌菌剂可以提高板蓝根和大青叶的质量。Objective： To improve the quality of Radix Isatidis and Folium Isatidis by applying Photosynthetic Bacteria.Methods： HPLC method was used for analyzing the content of Indirubin in Folium Isatidis with methanol-water（75：25） as the mobile phase and detecting wavelength at 289 nm.According to Pharmacopoeia,45% ethanol was used as solvent to detect the content of ethanol-soluble extractive in Radix Isatidis.Results： After using Photosynthetic Bacteria,the content of indirubin in Folium Isatidis increased from 0.11 mg/g to 0.26 mg/g,increased 136.4%.The content of ethanol-soluble extractive of Radix Isatidi increased from 42.2% to 47.2%,increased 11.8%.Conclusion： The quality of Radix Isatidis and Folium Isatidis can be improved by applying Photosynthetic Bacteria.

关 键 词：光合细菌 高效液相色谱法 板蓝根 大青叶 靛玉红 

分 类 号：R283[医药卫生—中药学]