均匀设计法优化北五味子愈伤组织诱导培养基研究  

Optimization of Inductive Callus Medium for Schisandga chinensis Baill by Uniform Design

作　　者：张佳[1] 金东淳[1] 金海林[1] 刘权钢[1] 

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《安徽农业科学》2011年第12期6977-6978,6987,共3页Journal of Anhui Agricultural Sciences

基　　金：国际合作研究项目

摘　　要：[目的]优化北五味子愈伤组织诱导培养基。[方法]采用均匀设计法,以诱导率为主要考察目标,对北五味子愈伤组织生长的激素成分进行多因素多水平考察。[结果]叶片和叶柄的最佳培养基组合均为MS+3.0 mg/L 6-BA;茎段为MS+3.0 mg/L 6-BA+0.6mg/L NAA。[结论]用均匀设计法优化愈伤组织诱导培养基省时、方便,且明显高于均匀设计试验方案中的诱导率。[Objective] To optimize the inductive medium for Schisandga chinensis Baill callus.[Method] By using uniform design method,with inductivity as main investigate target,the components of hormone for growth of Schisandga chinensis Baill callus were investigated from multi-factors and multi-levels.[Result] The optimum medium was MS＋3.0 mg/L 6-BA combination for tender leaf and petiole;the optimum medium was MS＋3.0 mg/L 6-BA＋0.6 mg/L NAA combination for stem.[Conclusion] Uniform design is a saving,convenient method for the optimum medium for callus,and more than that of the arrangement for the inductivity.

关 键 词：北五味子 愈伤组织 均匀设计 诱导率 

分 类 号：S567[农业科学—中草药栽培]