二次回归正交旋转组合设计在紫芝液体发酵培养条件优化中的应用  

Optimization of Liquid Fermentation Conditions of Ganoderma sinensis through Quadratic Orthogonal Rotation Combination Design

作　　者：任丹[1] 辜运富[1] 张小平[1] 田鸿[1] 

机构地区：[1]四川农业大学资源环境学院,四川雅安625014

出　　处：《西南农业学报》2011年第2期681-686,共6页Southwest China Journal of Agricultural Sciences

摘　　要：通过单因素试验和"二次回归正交旋转组合设计"方法,在液体培养条件下,研究了不同发酵条件对1株野生紫芝菌丝体产量的影响。建立了装液量、初始pH、转速、培养温度和接种量5个因素的正交回归模型:Y=2.85701+0.17458X1-0.19802X12-0.20552X22-0.19302X32-0.15802X42-0.12302X52。从模型推知,装液量90 mL,pH6.0,转速140 r/m,培养温度27℃,接种量10.5%时,紫芝菌丝达到最大生物量3.15 g,验证实验结果与模型值相符。In this paper,with liquid fermentation,both Single Factor Test and the Quadratic orthogonal Regressive Rotational Combination Test method were used to analyze the effect of different fermentation conditions on the mycelium biomass of one wild Ganoderma sinensis strain.The quadratic orthogonal regression model is of mycelium biomass（Y） to five factors of liquid volume（X1）,initial pH value（X2）,rotation speed（X3）,culture temperature（X4）,vaccinate amount（X5） and established as Y=2.85701＋0.17458X1-0.19802X21-0.20552X22-0.19302X23-0.15802 X24-0.12302 X25.It was concluded from the model that when liquid volume,initial pH value,rotation speed,culture temperature,vaccinate amount were 90 mL,6.0,140 r/min,27 ℃ and 10.5 % respectively,the mycelium biomass（Y） was the maximal（3.15 g） and consistent with the confirmatory experiment result.

关 键 词：紫芝 液体发酵 二次回归正交旋转 菌丝生物量 

分 类 号：S646[农业科学—蔬菜学]