人参总皂甙对白血病祖细胞作用的实验观察  

Experimental Observation of Function of Total Saponin of Ginseng to Leukemic Ancestral Cells

作　　者：高瑞兰 牛泱平 B.H.Chong 

机构地区：[1]浙江中医学院附属医院,浙江杭州310006 [2]澳大利亚新南威尔士大学

出　　处：《上海中医药杂志》1999年第9期46-48,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：浙江省自然科学基金 ;澳大利亚政府科研基金

摘　　要：采用白血病祖细胞集落形成（ＣＦＵＡＭＬ） 培养法，观察不同浓度人参总皂甙（ＴＳＰＧ） 对ＨＬ６０、Ｍｅｇ０１ 白血病细胞株和急性髓细胞白血病患者的骨髓细胞集落形成的影响，并以正常粒单祖细胞（ＣＦＵＧＭ）作比较。结果：正常和白血病祖细胞均对低浓度（５～２５ｕｇ／ｍｌ） 的ＴＳＰＧ有反应，集落数提高２６．１～３５．４％ ；中等浓度（７５ｕｇ／ｍｌ） 的ＴＳＰＧ仍然刺激正常ＣＦＵＧＭ 集落增加，但抑制ＨＬ６０、Ｍｅｇ０１ 和ＣＦＵＡＭＬ集落生长，抑制率分别为５０．８％ 、２５ ．１ ％ 和１９．０％ ；较高浓度（１００ｕｇ／ｍｌ） 的ＴＳＰＧ对白血病祖细胞的抑制作用更明显，ＨＬ６０ 不能形成集落，对Ｍｅｇ０１ 和ＣＦＵＡＭＬ的抑制率分别为５３．３％ 和２８ ．９ ％ ，而正常ＣＦＵＧＭ 仍生长良好。提示ＴＳＰＧ对白血病祖细胞的影响不同于正常祖细胞。Colony forming culture method of leukemic ancestral cells was used to observe influence of total saponin of ginseng(TSPG)in different concentration to colony formation of HL-60.Meg-01 leukemic clone and marrow cell(CFU-AML)in the patients with acute myelocytic leukemia and also compared with normal granular ancestral cells(CFU-GM).The results showed that both normal and leukemic ancestral cells reacted to TSPG in the lower concentration(5～25ug/ml)and colony unit enhanced to 26.1～35.4%.TSPG in the medium concentration(75ug/ml)still stimulated increase in normal CFU-GM colony but inhibited colony growth of HL-60,Meg-01 and CFU-AMI.The inhibitory rates were respectively 50.8%,25.1% and 19.0%.The inhibitory function of TSPG in the higher concentration(100ug/ml)to leukemic ancestral cells was more obvious and its inhibitory rates to Meg-01 and CFU-AML were respectively 53.3% and 28.9%.But,normal CFU-GM still grew quite well.These results indicate that influence of TSPG to leukemic ancestral cell is different from its influence to normal ancestral cells and TSPG in different concentration shows either the proliferative or inhibitory functions.

关 键 词：人参总皂甙 白血病祖细胞 中药 药理 

分 类 号：R286.91[医药卫生—中药学]