电针三阴交、血海对痛经模型大鼠子宫微循环的影响  

Effect of Electroacupuncture at Sanyinjiao and Xuehai on Uterine Microcirculation in Dysmenorrhea Rats

作　　者：赵雅芳[1,2] 李春华[1] 嵇波[1,2] 任晓暄[1,2] 郭孟玮[1,2] 苑鸿雯[1] 张露芬[1,2] 朱江[1,2] 

机构地区：[1]北京中医药大学针灸推拿学院,邮政编码北京100029 [2]国家中医药管理局针灸特色疗法评价重点研究室

出　　处：《微循环学杂志》2011年第2期4-7,I0001,F0002,共6页Chinese Journal of Microcirculation

基　　金：国家重点基础研究发展计划(973计划)课题(No.2006CB504503);国家自然科学基金面上项目(No.30973793);教育部博士点基金项目(No.20090013110005)

摘　　要：目的:观察电针三阴交、血海对原发性痛经(PD)模型大鼠子宫微循环的影响,进一步探讨针刺不同穴位缓解胞宫疼痛的差异和机制。方法:将24只动情间期雌性SD大鼠随机分为生理盐水组、PD模型组、电针三阴交组、电针血海组,每组6只。除生理盐水组外,其余各组大鼠均连续10天皮下注射苯甲酸雌二醇注射液,末次给药1h后,腹腔注射缩宫素0.2ml/只,制备痛经大鼠模型,生理盐水组每日给予同等剂量生理盐水。于第10天各电针组均行即刻电针20min。采用XW-B-3冷光源微循环显微检查仪观察各组大鼠电针5min、10min、20min时子宫外膜微循环的变化。结果:与生理盐水组比较,PD模型组微血管、毛细血管(Cap)粗细不均,管径收缩,微血管、Cap计数减少,血流减慢或停滞。与PD模型组比较,即刻电针三阴交5min时,Cap管径显著扩张(P<0.05),电针20min时,微血管、Cap管径均显著扩张(P<0.01,P<0.05),Cap计数显著增多(P<0.05);即刻电针血海各时间点的各项指标均无显著性差异(P>0.05)。与血海组比较,电针三阴交20min时,Cap计数显著增多(P<0.05)。结论:即刻电针可缓解子宫血管的痉挛状态,改善子宫微循环,缓解胞宫疼痛,且经穴之间存在一定差异,三阴交优于血海。Objective:To observe the effect of electroacupuncture(EA)at Sanyinjiao(SP6)and Xuehai(SP10)on uterine microcirculation in dysmenorrhea rats in order to discuss the mechanism of acupuncture in relieving uterine pain,to discuss the specificity of acupoints preliminarily.Method:Twenty-four Sprague-Dawley(SD)diestrus female rats were randomly divided into saline group,model group,EA SP6 and EA SP10,each group had 6 rats.Estradiol benzoate and oxytocin were chosen to be the model of dysmenorrhea.Except saline group,other groups were given subcutaneous estradiol benzoate injection,after the last administration 1h,intraperitoneal injection of oxytocin 0.2ml/each.Saline group was given the same dose of saline per day,on the 10th day,treatment groups were given instant EA for 20 minutes.Using XW-B-3 cold light micro-circulation tester to observe the changes of uterus microcirculation at different times.Results:Compared with saline group,the diameter of micro-vessel and cap in model group were shrinked,numbers of micro-vessel and cap were reduced,the blood flow was slow and even stopped;compared with model group,EA SP6 for 5 minutes,diameter of cap was significantly expanded(P<0.05),EA SP6 for 20 minutes,diameters of micro-vessel and cap were significantly expanded(P<0.01,P<0.05),number of cap was significantly increased(P<0.05),the state of blood flow was not significant difference(P>0.05).There wasn't significant difference in each indicator after EA SP10(P>0.05);compared with SP10,EA SP6 for 20 minutes,cap number was remarkably expanded(P<0.05).Conclusion:EA can relieve spasm of uterus blood vessels,improve micro-circulation and relieve pain,there were differences among acupoints,SP6 was better than SP10.

关 键 词：痛经 子宫微循环 三阴交 血海 

分 类 号：R245.9[医药卫生—针灸推拿学]