水牛催乳素基因第3外显子群体遗传特征分析  

Genetic Characteristics on the Exon 3 of Prolactin Gene in Buffalo(Bubalus bubalus)

作　　者：袁峰[1] 苗永旺[1,2] 李大林 刘丽仙[4] 熊飞[1] 刘伟[1] 贡潘偏抽[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南大学云南省生物资源保护与利用重点实验室,云南昆明650091 [3]云南巴富乐水牛技术研究所,云南昆明650021 [4]云南农业职业技术学院畜牧兽医系,云南昆明650212

出　　处：《云南农业大学学报（自然科学版）》2011年第3期312-316,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省应用基础研究重点项目(2007C0003Z);国家自然科学基金项目(30660024);云南省应用基础研究计划面上项目(2006C0034M;2010ZC243);国家高技术研究发展计划("863"计划)项目(2008AA101001);云南省教育厅科学研究基金项目(2010Y344;09C0197)

摘　　要：催乳素基因(PRL)第3外显子(exon 3)对奶牛产奶量、乳脂产量和乳蛋白产量有显著影响,但在水牛中该基因的遗传特征及其与产奶性状是否有遗传关联目前还不清楚。本研究采用PCR-SSCP并结合PCR产物直接测序技术,分析了沼泽型水牛和河流型水牛共12个群体275个个体PRL exon 3的遗传变异,结果表明:水牛PRL exon 3由108个核苷酸组成,编码36个氨基酸,在水牛群体中未检测到单核苷酸多态性位点(SNPs),PRL exon 3与水牛产奶量之间没有直接相关性。通过序列比对发现在不同物种中PRL exon 3具有高度保守性。但在PRL第2内含子(intron 2)的2 265 nt和2 335 nt分别检测到G>A突变和C>T突变,其中2 335 nt位点突变仅在2头槟榔江水牛中检测到。The exon 3 of prolactin gene had significant effect on yield of milk, milk fat and milk protein in cow, but the genetic characteristics and the relevance between milk production traits and gene mutation on the exon 3 of prolaetin gene in water buffalo are unclear. In the present study, the sequence features of the exon 3 in prolactin gene were examined in 275 individuals which came from 12 river and swamp type buffalo populations using PCR-SSCP and PCR production direct sequencing methods. The results showed that the sequence of exon 3 in prolactin gene was consists of 108 nucleotides, and the sequence coding for 36 amino acid residues, the SNPs loci wasn＇t detected in water buffalo, so there was irrelevance between milk yield traits and the exon 3 of prolactin gene in water buffalo. The fragment had high conservative character in different species by sequences alignment. Two substitution was detected in intron 2 of prolactin gene, one is G 〉 A substitution at the 2 265 th nucleo- tide and another is C 〉 T substitution at the 2 335 th nucleotide. Moreover, the C 〉 T substitution at the 2 335 th nucleotide was only detected in 2 Binlangjiang water buffaloes.

关 键 词：河流型水牛 沼泽型水牛 催乳素 EXON 3 PCR—SSCP 

分 类 号：S823.83[农业科学—畜牧学]