地高辛标记寡核苷酸探针对慢性乙肝患者前 C基因突变的检测  

DIGOXIN TAGGED OLIGONUCLEOTIDE PROBES DETECT CHRONIC HEPATITIS B PATIENTS WITH PRECORE REGION GENE MUTATION

作　　者：王晓燕[1,2] 曲春枫[3] 王延军[3] 玄恩余[4] 苗乃法[3] 郭少愚[5] 李在连[3] 

机构地区：[1]山东医科大学微生物学教研室 [2]山东济南 250012 [3]潍坊医学院免疫学教研室 [4]潍坊市人民医院传染科 [5]东营市胜利油田中心医院传染科

出　　处：《潍坊医学院学报》1999年第3期171-173,共3页Acta Academiae Medicinae Weifang

摘　　要：目的　研究慢性乙肝患者乙肝病毒（ＨＢＶ）前Ｃ区突变情况。方法　用多聚酶链反应（ＰＣＲ）结合地高辛标记的寡核苷酸探针Ｍ０（野毒株）、Ｍ１（１８９８位变异株）、Ｍ２（１８９８／１９０１位变异株）对１６５例慢性乙肝患者前Ｃ区突变情况进行检测。结果　其中１２例ＨＢｓＡｇ（＋），ＨＢｅＡｇ（＋），抗ＨＢｅ（－），抗ＨＢｃ（＋）患者，ＨＢＶＤＮＡ阳性率为１００％，突变株检出率为８．３％。１５３例ＨＢｓＡｇ（＋），ＨＢｅＡｇ（－），抗ＨＢｅ（＋），抗ＨＢｃ（＋）患者，ＨＢＶＤＮＡ总阳性率为１６．３％，突变株总检出率为８４％。结论　前Ｃ基因突变株主要存在于抗ＨＢｅ阳性的慢性乙肝患者体内，ＰＣＲ结合地高辛标记的寡核苷酸探针杂交技术，操作简便快捷，利于临床推广应用。Objective To study HBV precore region mutation in chronic hepatitis B patients of Shandong province.Method By using digoxin tagged oligonucleotide probes M 0(wild type),M 1(mutated at nt 1898),M 2(mutated at nt 1898/1901) to detect the PCR products of HBV pre C and C gene region in 165 cases of chronic hepatitis B patients in Shandong province.Result In 12 cases of patients with HBsAg(+),HBeAg(+),anti HBe(-),anti HBc(+),the positive rate of HBV DNA was 100%( 12/12),the rate of precore mutations was only 8.3%( 1/12).In 153 cases of patients with HBsAg(+),HBeAg(-),anti HBe(+),anti HBc(+),the positive rate of HBV DNA was 16.3%(25/153),the rate of precore mutations was 84%(21/25).Conclusion These results suggest that precore mutations mainly prevail in chronic hepatitis B patients with serum HBeAg(-),anti HBe(+),the method of using digoxin tagged oligonucleotide probes to detect the precore mutated region is easy and simple,it could be applied to clinical practice widely.

关 键 词：乙型肝炎病毒 前C基因突变 PCR 乙型肝炎 

分 类 号：R512.620.4[医药卫生—内科学]