毛细管电泳法测定苹果中维生素含量  

Determination of vitamin in apples by capillary zone electrophoresis

作　　者：曹慧[1] 刘海兴[1] 张玉宵[1] 

机构地区：[1]潍坊学院生物工程学院,山东潍坊261061

出　　处：《实验技术与管理》2011年第4期31-32,共2页Experimental Technology and Management

基　　金：潍坊市科技发展计划项目资助(2009038);生命分析化学教育部重点实验室开放基金(KLACLS1002);潍坊学院优秀学术团队项目(2008Z01)

摘　　要：采用毛细管区带电泳法测定了5种苹果中维生素C和烟酸的含量。在20mmol/L硼砂和20%乙腈的混合缓冲体系(pH 8.5)中,两种维生素在15min内较好地得到分离。维生素C和烟酸的迁移时间和峰面积的相对标准偏差分别为5.43%和2.35%、6.24%和2.49%。质量浓度在0.007 81～1 000mg/L范围内线性相关系数分别为0.9964、0.9945。该方法简单、准确,可作为果实品质测定的一种方法。Vc and nicotinic acid of five kinds of apples were directly determined by capillary zone electrophoresis.Under the mixed electrolyte（pH8.5）,which was made of 20mmol L-1 borate and 20% ethyl,both of them were well separated within 15 minutes.The RSDs of migration time were 5.43%,6.24%,and its peak areas were 2.35%,2.49%,respectively.Correlation coefficients were 0.9964 and 0.9945 within the concentration range from 0.0078125mg·mL-1 to 1.0mg·mL-1.The method was simple and accurate as a new method to determine fruit quality.

关 键 词：苹果 维生素C 毛细管区带电泳 烟酸 

分 类 号：S661.133[农业科学—果树学]