小鼠膜性肾小球肾炎的形态学计量研究  

Quantitative study of morphological images on membranous glomerulonephritis of mice

作　　者：张虎祥[1] 张海燕[2] 万丽[1] 杨开颜[1] 

机构地区：[1]温州医学院附属第一医院病理科,温州325000 [2]温州医学院附属第一医院急诊科,温州325000

出　　处：《中国临床保健杂志》2011年第2期176-178,共3页Chinese Journal of Clinical Healthcare

基　　金：温州市科技局基金(Y20090041)

摘　　要：目的通过光镜、电镜定量研究分析小鼠膜性肾小球肾炎(MGN)的病理变化。方法制备小鼠MGN模型,对模型小鼠肾小球进行光镜、电镜形态学观察,并予定量分析。结果光镜、电镜观察均显示病理组具有典型的MGN病变。病理组基底膜(GBM)厚度分别为0.273nm,对照组为0.1354nm,病理组与对照组比较,差异有统计学意义(P<0.01)。病理组肾小球的平均直径(D—),平均周长(B—),平均体积(V—),平均截面积(A—),以及体密度(Vv),面密度(Sv)均大于对照组(P<0.05或P<0.01)。数密度(Nv)与比表面(δ)则相反,对照组大于病理组(P<0.01)。结论光镜、电镜定量研究较为客观地反映肾小球肾炎小鼠肾小球的病变程度,为肾小球肾炎发病机理研究及临床治疗提供有价值的参考。Objective The pathological changes of membranous glomerulonephritis（MGN）in mices were analysed.Method MGN model in mice was established.The pathological changes were observed and quantitative study by light microscope（LM）and electronic microscope（EM）.Result Observation showed that pathology group has typical MGN pathological changes.Quantitative study of LM and EM images showed that pathology group were remarkable different from those of the control group.Conclusion Quantitative analysis could objectively reflex the glomerulus pathological changes of glomerulonephritis,and provide some reference for the study of pathologenesis、clinical diagnosis and therapy of mice glomerulonephritis.

关 键 词：肾小球肾炎 膜性 显微镜检查 小鼠 近交BALB/C 

分 类 号：R692.31[医药卫生—泌尿科学] R44[医药卫生—外科学]