彗星试验中两种染毒条件的对比研究  

A COMPARATIVE STUDY OF TWO EXPOSURE CONDITIONS IN COMET ASSAY.

作　　者：张遵真[1] 衡正昌[1] 王涛[1] 

机构地区：[1]华西医科大学环境卫生教研室,成都610041

出　　处：《现代预防医学》1999年第3期334-335,共2页Modern Preventive Medicine

摘　　要：本文对彗星实验的悬浮染毒与传统染毒方式进行了敏感性和方法学的对比研究。结果表明：采用悬浮染毒检测化合物引起的DNA损伤比传统染毒方式具有更敏感、节省细胞和时间、均匀性好、操作更方便等优点，值得推广。文中还对悬浮染毒方式的某些实验条件进行了探讨。in this paper, two exposure conditions were compared in comet assay. The results revealed that the method of suspended exposure was more sensitive than that of traditional exposure for detecting DNA damage of chemicals and needed less cellsas well as easier to manipulate. Some experimental conditions for the method of suspended exposure were also discussed.

关 键 词：彗星实验 暴露方式 DNA损伤 

分 类 号：R99[医药卫生—毒理学]