知母中萨尔萨皂甙元的气相色谱定量测定  

Determination of Sarsasapogenin in Anemarrhena asphodeloides Bunge by GC

作　　者：刘垣升[1] 陈万生[1] 乔传卓[1] 赵宁志[2] 

机构地区：[1]第二军医大学,上海200433 [2]南京军区后勤部,南京210003

出　　处：《中国中药杂志》1999年第9期554-555,共2页China Journal of Chinese Materia Medica

摘　　要：目的:测定知母根茎中萨尔萨皂甙元的含量。方法:采用毛细管气相色谱法,以胆固醇为内标,样品在柱温为270℃的HP1色谱柱上,以氮气作载气进行分析。结果和结论:在0.245～2.94μg·μl-1线性良好,平均回收率为95.52%±1.77%。Objective:To determine sarsasapogenin in Anemarrhena asphodeloides .Method:Chloromethane extract(1μl) with cholesterol as internal standard was analyzed on HP 1 column, operated at 270℃ with N 2 as carrier gas and FID.Result:The method was linear within the range of 0.245～2.94μg·μl -1 ,with a correlation coefficient of 0.9996. The average recovery was 95.52%±1.77%,Conclusion:The method is reproducible, rapid and sensitive.

关 键 词：知母 萨尔萨皂甙元 气相色谱法 中药 测定 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]