不同强度电针对肥胖大鼠脂肪组织炎症相关因子的影响  

Effect of Different Intensities of Electroacupuncture on the Inflammatory Factor in Adipose Tissues from High Fat Diet-induced Obese Rats

作　　者：余敏[1] 唐成林[2] 刘祖丽[1] 侯懿烜[1] 郜婕[1] 刘仁建[1] 

机构地区：[1]重庆医科大学中医药学院 [2]重庆医科大学科研处,中国重庆400016

出　　处：《生命科学研究》2011年第2期142-146,共5页Life Science Research

基　　金：重庆市卫生局资助项目(07-2-63);重庆市九龙坡区科委资助项目(06370302)

摘　　要：探讨不同强度电针对肥胖大鼠脂肪组织核因子-κBp65(NF-κBp65)、单核细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)的作用差异.将SD大鼠随机分为普通饮食组、高脂饮食组、5 V电针组、2.5 V电针组,除普通饮食组外其余各组大鼠均饲以高脂饲料.取"足三里"、"三阴交"穴,不同强度电针治疗14 d后,用蛋白质印迹技术(Western blot)检测肥胖大鼠附睾脂肪组织NF-κBp65的表达,酶联免疫吸附法(ELISA)检测肥胖大鼠附睾脂肪组织MCP-1、TNF-α的含量.研究发现两电针组肥胖大鼠体重、Lee’s指数、脂肪组织中NF-κBp65表达、MCP-1和TNF-α含量较高脂饮食组显著降低(P<0.01),5 V电针组较2.5 V电针组下降效果更为明显(P<0.01,P<0.05).结果表明电针可改善肥胖脂肪组织炎症反应状态,减轻肥胖大鼠体重,且5 V电针组效果优于2.5 V电针组.To investigate the effect differences with different intensities of electroacupuncture（EA） on the expression of NF-κBp65,MCP-1 and TNF-α in adipose tissue from high fat diet-induced obese rats.SD rats were randomly devided into common diet group（CD）,high fat diet group（HFD）,high fat diet with 5 V EA group（5 V EA） and high fat diet with 2.5 V EA group（2.5 V EA）.Obesity was induced by feeding the rats with high fat diet.EA（frequency： 20 Hz,intensities： 5 V and 2.5 V）,was applied to＂Zu san li＂,＂San yin jiao＂for 15 min everyday and for 14 days.Body weight and Lee＇s index in each group were detected.The expression of NF-κBp65 in epididymis adipose tissue was detected by Western blot,the contents of MCP-1 and TNF-α in adipose tissue was detected by ELISA.Body weight,Lee＇s index,the expression of NF-κBp65MCP-1 and TNF-α from obese rats of two EA groups were significantly decreased（P 0.01） compared with HFD group.5 V EA group exhibited a better improvement on the protein expression with the 2.5 V EA group（P 0.01,P 0.05）.The results indicated that EA can improve adipose tissue inflammatory response with simple obesity,reduce body weight of the obese rats,moreover,5 V EA group is superior to 2.5 V EA group.

关 键 词：电针 肥胖 附睾 脂肪组织 

分 类 号：R245.97[医药卫生—针灸推拿学]