转青鱼生长激素基因日本白鲫原代的研制  

The Development of P_0 of Black Carp GH gene Transgenic Japanese Crucian Carp

作　　者：冯浩[1] 傅永明[1] 吴慧[1] 刘筠[1] 刘少军[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》2011年第2期158-164,共7页Life Science Research

基　　金：国家公益性行业(农业)课题(200903046-08);国家自然科学基金资助项目(30500294);湖南师范大学青年优秀人才培养计划资助项目(ET31004)

摘　　要：构建由青鱼β-actin基因启动子和青鱼生长激素(GH)基因精确拼接的"全鱼"基因pbcAbcGHc,并采用显微注射法将pbcAbcGHc导入日本白鲫的受精卵中.对照养殖结果显示150日龄P0代转基因日本白鲫的平均体重是对照组1.37倍,平均体长是对照组1.07倍.选择30尾P0代转基因日本白鲫,PCR方法检测出外源青鱼GH基因在P0代转基因日本白鲫尾鳍基因组DNA中的整合率为90%;在一尾生长速度显著的转青鱼GH基因日本白鲫P0的肌肉、肝脏两种组织中可检测到外源青鱼GH基因的转录.P0代转青鱼GH基因日本白鲫P0的成功获得为建立转青鱼GH基因日本白鲫纯系奠定了基础.The＂all fish＂ transgene（pbcAbcGHc） containing the promoter of the β-actin gene and cDNA of growth hormone（GH） gene of black carp was microinjected into fertilized eggs of Japanese crucian carp（JCC）.The contrast cultivation results showed that the mean body weight of the P0 of transgenic JCC of 150 days was 1.37 times of the control group.The average body length of the P0 of transgenic JCC of 150 days was 1.07 times of the control group.30 transgenic JCC of 150 days were chosen for tail fin genomic DNA isolation and subsequently PCR detection.The ratio of the exogenous transplant gene integrating in the genome of the JCC tail fin is 90%.The transcription of exogenous black carp GH gene could be detected in the total RNA of the muscle and liver from one JCC with outstanding growth rate.The P0 of transgenic Japanese crucian carp with higher growth rate had been successfully developed,which made the solid foundation for the establishment of the pure line of the transgenic JCC.

关 键 词：转基因 日本白鲫 生长激素 

分 类 号：S961.6[农业科学—水产养殖]