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作 者:李丽[1] 张锦韬[2,3] 段凤云[4] 周冀衡[2] 郭春秋[1] 胡海燕[1]
机构地区:[1]湖南文理学院生命科学学院,湖南常德415000 [2]湖南农业大学烟草工程研究中心,长沙410128 [3]湖南中烟工业有限责任公司原料部,长沙410007 [4]云南省烟草公司大理州公司生产部,云南大理654600
出 处:《中国烟草科学》2011年第2期29-32,共4页Chinese Tobacco Science
摘 要:利用选择性培养基,采用最大或然数法对其计数,对云南大理州主要植烟区土壤硫化细菌及反硫化细菌进行分离,以了解硫化细菌和反硫化细菌在烟草不同生长时期的动态变化。结果表明,剑川甸南、下庄、程官和弥渡4个烤烟主产区硫化细菌和反硫化细菌根际土细菌数大于田间土,硫化细菌根际与田间土之比为R:S=6~10:1,反硫化细菌的根土比约R:S=2.5~3.5:1,弥渡烤烟区细菌数量要明显大于其他3个烤烟区。永平和南涧地区田间土硫化细菌数比根际要高。土壤pH与土壤细菌含量呈负相关性,对反硫化细菌的影响要远远大于硫化细菌;根际硫化细菌与田间土硫化细菌呈显著正相关性r=1.00,根际与田间土反硫化细菌呈显著正相关性r=0.879。硫化细菌在成熟期含量较高,反硫化细菌数在团棵期含量较高。In order to find out dynamic change of sulfur bacteria and anti-sulfide bacteria at different tobacco growth stage in Dali,Yunnan province,the major sulfur bacteria and anti-sulfide bacteria were separated by use of selective medium.The results showed that at Jianchuan,Xiazhuang,Midu and Chengguan,the four main flue-cured tobacco producing areas,the number of sulfur and anti-sulfide bacteria of rhizosphere were more than that of field soils,and the proportion were R:S = 6-10:1 and R:S = 2.5-3.5:1 respectively.However,the field soil sulfur bacteria quantity of Yongping and Nanjian was more than that of rhizospheric soil.The number of bacteria in Midu was significantly larger than the other three areas.pH value was negatively related to the quantity of bacterial,and its influence on anti-sulfur bacterial was much larger than that of sulfur bacteria.Rhizospheric soil sulfur bacteria and field sulfur bacteria was significantly positive correlation(r=1.00),and rhizospheric anti-sulfur bacteria was positively correlate to field soil Anti-bacteria(r=0.879).Sulfur bacteria kept higher level at maturity stage,but the highest level of anti-sulfur bacteria appeared at the rosette stage.
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