肾血管性高血压大鼠运动区 N P Y 阳性神经元的变化  

Changes of NPY Neurons in the Motor Area in Renovascular Hypertensive Rats

作　　者：王省[1] 高凤兰[1] 董华丽[1] 原志庆[1] 文小军 

机构地区：[1]新乡医学院解剖学教研室河南新乡,453003 [2]河南漯河卫校病理教研室

出　　处：《高血压杂志》1999年第3期282-284,共3页Chinese Journal of Hypertension

摘　　要：目的：研究肾血管性高血压大鼠运动区 Ｎ Ｐ Ｙ阳性神经元的变化。　方法：雄性 Ｓ Ｄ大鼠 ６０ 只，随机分为两组（ｎ＝ ３０）。手术组用单夹肾动脉法复制高血压模型，对照组手术过程同手术组，但不上银夹。于术后 ７ ｄ，３０ ｄ，９０ ｄ 处死动物，取脑，用 Ａ Ｂ Ｃ免疫组化方法对大鼠运动区 Ｎ Ｐ Ｙ 阳性神经元进行定量分析。　结果：对照组运动区 Ｎ Ｐ Ｙ阳性神经元密度随年龄增加而减少，而手术组 Ｎ Ｐ Ｙ阳性神经元密度先减少，后增加，最后保持不变。手术组和对照组 Ｎ Ｐ Ｙ 神经元密度相比，术后 ７ ｄ 两组间无显著差异；术后 ３０ ｄ 手术组小于对照组（ Ｐ＜ ０．０５）；术后 ９０ ｄ 手术组大于对照组（ Ｐ＜ ０．０１）。　结论： Ｎ Ｐ Ｙ阳性神经元在高血压的发生、发展过程中起一定调控作用。Purpose:To research the changes of NPY neurons in the brain motor area in renovascular hypertensive rats. Methods:Sixty male SD rats were divided randomly into operated group and sham control group ( n =30). In operated rats, renal arteries were constricted bilaterally with silver clips(0.3 mm in internal diameter)to induce RHR. 7 d, 30 d, 90 d after operation the rats were killed, their brains were removed and analysed by ABC immunohistochemistry in the motor area. Result: In control sham group,the density of NPY positive neurons in the motor area decrease with the increase of age, whereas those of the operated group the NPY positive neurons decrease first and then increase, then after remain relatively constant. Comparing the density of NPY of operated group with control group, in the early period(7 d after operation), no significant difference has been found in the density of NPY neuron; in the middle period(30 d after operation) the density of NPY neurons in operated group are lesser than that of control group ( P <0.01); in the late period(90 d after operation), the density of NPY neuron in operated group are greater than control group ( P <0.05). Conclusions:NPY neurons system might mediate the development of hypertension.

关 键 词：神经肽Y 免疫组化 运动区 高血压 病理 

分 类 号：R544.102[医药卫生—心血管疾病]