基于线粒体细胞色素b基因的3个兰州鲇群体的遗传多样性分析  

Genetic diversity analysis of three groups of Silurus lanzhouensis based on mitochondrial DNA cytochrome b gene sequences

作　　者：肖伟[1] 侯玉霞[2] 蔡原[1] 连总强 吴旭东 

机构地区：[1]甘肃农业大学动物科技学院,甘肃兰州730070 [2]中国农业大学理学院,北京100193 [3]宁夏回族自治区水产研究所,宁夏银川750001

出　　处：《甘肃农业大学学报》2011年第2期11-16,共6页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(30760191);宁夏自然科学基金项目(NZ08164;NZ09184)

摘　　要：测定了3个兰州鲇群体,即野生兰州鲇（WS）、兰州鲇亲本（QS）、人工繁育鲇子一代（ZS）,共56尾样本的线粒体细胞色素b基因,运用MEGA 4.0、DNASP等软件对样本的968 bp线粒体Cytb基因序列进行了分析。结果显示：共检测到17种单倍型、99个变异位点,其中有93个是多态位点（包含74个简约信息位点）,总变异率为10.23%;对同源序列碱基组成分析显示,T、C、A、G碱基的平均含量分别为26.87%、29.46%、29.16%、14.53%,A＋T含量大于G＋C含量;Kimura′s 2-parameter模型分析得到兰州鲇3个群体之间的遗传距离介于0.001 1~0.084 4之间,兰州鲇3个群体（QS、ZS、WS）单倍型多样性指数分别是（0.916±0.027）、（0.714±0.071）、（0.857±0.039）,核苷酸多样性指数分别（0.008 01±0.000 006 9）、（0.002 87±0.000 001 2）、（0.019 55±0.000 025 0）.In this study,the mitochondrial DNA cytochrome b of 56 samples representing three groups of Silurus lanzhouensis were sequenced.The MEGA 4.0 and DNASP software were used to analyze the 968 bp gene fragment.The results showed that：17 haplotypes,99 sites of mutation were detected accounting for 10.23 % of total sequence,93 sites were polymorphism sites,in which 74 sites were parsimony informative sites.After being aligned,the average basic compositions of all sequences were as follows：26.87 % A,29.46 % C,29.16 % T and 14.53 % G,while the average basic compositions of A＋T were bigger than G＋C.Genetic distance were calculated by the Kimura′s 2-parameter model,which ranged from 0.001 1 to 0.084 4,haplotype diversity of three groups of Silurus Lanzhouensis（QS,ZS and WS） were（0.916±0.027）,（0.714±0.0710） and（0.857±0.039）,respectively,and nucleotide diversity of those three groups were（0.008 01±0.000 006 9）,（0.002 87±0.000 001 2） and（0.019 55±0.000 025 0）,respectively.

关 键 词：兰州鲇 细胞色素B基因 遗传多样性 

分 类 号：S965.128[农业科学—水产养殖]