猪场环境大肠埃希菌耐药质粒消除研究  

Study on Elimination of Drug-resistance Plasmid of E.coli

作　　者：李基棕[1] 周碧君[1,2] 马喆[1] 彭全海[1] 高颖[1] 张人俊[1] 王开功[1,2] 文明[1,2] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025

出　　处：《动物医学进展》2011年第4期36-40,共5页Progress In Veterinary Medicine

基　　金：贵州省科技厅农业科技攻关项目(黔科合NY字[2009]3069号)

摘　　要：为了解耐药质粒消除对大肠埃希菌耐药性及其他生理特性的影响,参考前期试验分离出的8株携带aac(6’)-Ib质粒耐药基因的大肠埃希菌的药敏试验。选取1株耐药性最强菌株用高温及SDS进行耐药质粒消除,PCR检测aac(6’)-Ib基因。结果显示,耐药质粒成功消除,耐药性消失,且细菌在不含抗生素条件下培养,耐药性消除状态可稳定遗传;但在含卡那霉素条件下连续传代,其耐药性可迅速恢复,第4代即可产生耐药性。体外生长曲线显示,耐药质粒消除株生长较快,且较早进入对数期。以上数据表明大肠埃希菌多重耐药性主要由质粒决定,研究开发新型耐药质粒消除剂对克服大肠埃希菌耐药性具有重要意义。To understand the drug resistance and the other physiological property after plasmid elimination treatment of E.coli.The E.coli with plasmid containing aac（6＇）-Ib gene was used for drug sensitivity test.The drug sensitivity showed that the strains almost resisted all of the 8 antibiotics including Aminoglycosides and Quinolones.In this study one strain of highest drug-fast was reversed by high temperature and SDS.PCR results showed that the resistant gene named aac（6＇）-Ib was eliminated,and the drug resistance was disappeared.When the strain was cultured without antibiotic,the drug sensitivity was constant.But the environment of kanamycin could recover the drug resistance after 4 generations.The strain without drug-resistance plasmid growed faster,and went into logarithmic phase earlier.Therefore,drug resistance of E.coli caused by plasmid.The strategy to eliminate the plasmids would be an effective way to deal with the multiresistance issue.

关 键 词：大肠埃希菌 耐药质粒 质粒消除 

分 类 号：S852.615[农业科学—基础兽医学]