MCP-1﹑Ⅳ胶原蛋白和血清对单核细胞迁移作用的实验研究  

Effects of MCP-1、collagen Ⅳ and serum on monocyte migration

作　　者：王萍[1] 吴静[2] 徐锦堂[2] 丁姗姗[2] 侯光辉[3] 

机构地区：[1]暨南大学附属第一医院眼科,广州510630 [2]暨南大学医学院眼科研究室,广州510632 [3]广东省珠海市人民医院眼科,519000

出　　处：《广东医学》2011年第7期840-842,共3页Guangdong Medical Journal

基　　金：暨南大学侨办重点学科基金资助项目(编号:51205004);广东省珠海市科技计划项目(编号:PB20051014)

摘　　要：目的初步探讨单核细胞趋化蛋白-1(MCP-1)﹑Ⅳ型胶原蛋白(Ⅳcollagen)和血清在单核细胞迁移过程中的作用。方法体外单核细胞迁移模型的建立,在体积分数10%FBS/1μgⅣcollagen和体积分数0%FBS/1μgⅣcollagen的实验条件下添加650 ng/mL MCP-1与0 ng/mL MCP-1。流式细胞术检测迁移前后单核细胞表面抗原CD14阳性率。结果在体积分数0%FBS/1μgⅣcollagen的实验条件加入650 ng/mL MCP-1时细胞生长良好。迁移前CD14细胞阳性率19.6%,迁移后CD14细胞阳性率1.5%。结论单核细胞在FBS体积分数为0%,Ⅳcollagen浓度为1μg、MCP-1浓度为650 ng/mL实验条件时细胞迁移数量较多。Objective To investigate the effects of MCP - 1, collagen IV and serum on monocyte migration. Methods To establish the monocyte migration model in vitro. MCP - 1 at the concentration of 650 ng/mL or 0 ng/mL was added into FBS in volume fraction 10% and 0% with 1 μg collagen IV for stimulation. CD14 on monocytes was detec- ted by flow cytometry before and after migration. Results CD14 positive rates of monocytes were 19.6% and 1.5% be- fore and after migration, respectively. Significantly better growth was observed on monocytes with I p.g collagen Ⅳ at 650 ng/mL of MCP - 1 and FBS in 0% volume fraction. Conclusion Monocytes in 1 μg collagen IV at 650 ng/mL MCP - 1 with FBS in 0% volume fraction present maximal migration ability.

关 键 词：单核细胞 迁移模型 MCP-1 Ⅳcollagen 血清 

分 类 号：R722.12[医药卫生—儿科]