分子标记辅助选择改良武运粳8号的条纹叶枯病抗性  

Improving the Resistance of Wuyunjing 8 to Rice Stripe Virus via Molecular Marker-assisted Selection

作　　者：张宏根[1] 许作鹏[1] 李鹏[1] 李波[1] 刘超[1] 朱邦辉 徐洁芬 钮中一 汤述翥[1] 梁国华[1] 顾铭洪[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009 [2]江苏省常州市武进区农业科学研究所,江苏常州213175

出　　处：《作物学报》2011年第5期745-754,共10页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2006CB101701;2006CB101703);国家转基因生物新品种培育重大专项(2008ZX08001-002)资助

摘　　要：本研究旨在改良武运粳8号的条纹叶枯病抗性。2004年,在江苏扬州对江苏省1981—2002年间审定的25个迟熟中粳品种进行产量鉴定,从中筛选出直立穗高产品种武运粳8号作为条纹叶枯病抗性改良的受体亲本。利用抗条纹叶枯病品种葵风为供体亲本,通过杂交和回交,同时利用4个与条纹叶枯病抗性基因紧密连锁的分子标记STS11-31、STS11-71、STS11-19和STS11-43进行辅助选择,至2008年正季,共计获得70个BC3F5以及115个BC4F4抗条纹叶枯病的稳定株系。经回交后代农艺性状、产量性状、品质性状和抗性的系统鉴定,从中筛选出10个BC4F5株系和2个BC3F6株系,这些株系综合性状与武运粳8号已十分相近,保持了武运粳8号的丰产性和优良品质,明显提高了条纹叶枯病的抗性。In this study,a molecular marker-assisted selection （MAS） strategy was used to improve Wuyunjing 8＇s resistance to rice stripe virus.The Wuyunjing 8 with high yield and erect head selected from 25 late-maturing varieties was used as accepter,and the variety Kuifeng with resistance to rice stripe virus as the donor.Four STS （sequence-tagged site） primers STS11-31,STS11-7,STS11-19,and STS11-43 anchoring the resistance genes were used for assisted selection in the backcross progenies.To 2008,seventy lines in BC3F5 and one hundred and fifteen lines of BC4F4 with stable agronomic traits were obtained with the result of molecular analysis.By appraising agronomic traits,yield,quality and resistance in backcross progenies,teen lines from BC4F5 and two lines from BC3F6,which all carried disease-resistant genes and were similar to Wuyunjing 8 on the integrated traits,were selected.These lines retain the characteristics of high yield and good quality of Wuyunjing 8 and have been prominently improved in resistance to rice stripe virus.

关 键 词：粳稻 条纹叶枯病 抗性 改良 分子标记辅助选择 

分 类 号：S511.22[农业科学—作物学]