水稻抗稻曲病数量性状座位及效应分析  

Analysis of Quantitative Trait Loci for Resistance to Rice False Smut

作　　者：李余生[1] 黄胜东[1] 杨娟[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心,江苏南京210014

出　　处：《作物学报》2011年第5期778-783,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31071397);江苏省自然科学基金项目(BK2009320);国家高技术研究发展计划(863计划)项目(2010AA101302);江苏省农业科技自主创新基金项目(CX[10]403)资助

摘　　要：利用157个家系组成的大关稻(japonica)/IR28(indica)重组自交系(recombinant inbred lines,RIL)群体,采用高效引发稻曲病人工接种方法,以病情指数作为稻曲病的表型值。2007年和2009年,鉴定亲本及RILs对水稻稻曲病的抗性。利用QTL Cartographer软件,对水稻稻曲病抗性基因进行检测分析。两年检测到qFsr1、qFsr2、qFsr4、qFsr8、qFsr10、qFsr11、qFsr12共7个QTL,分别位于第1、第2、第4、第8、第10、第11和第12染色体上,贡献率在9.8%～22.5%之间。其中,2007年检测到qFsr1、qFsr4、qFsr10、qFsr11、qFsr12共5个位点;2009年检测到qFsr2、qFsr8、qFsr10、qFsr11共4个位点,qFsr10、qFsr11在两年中均被检测到,对性状的解释率在18.0%～19.3%之间,使病情指数下降8.0%～16.3%,提高了抗病性。根据抗性位点加性效应方向,在qFsr1、qFsr2、qFsr8、qFsr10、qFsr11和qFsr12位点上,亲本IR28存在抗稻曲病的增效等位基因,大关稻具有减效等位基因,而位点qFsr4的抗性效应来源正好相反。qFsr11、qFsr12及其附近的标记可望在稻曲病抗性分子标记辅助选择育种中加以应用。A recombinant inbred line （RILs） population with 157 lines derived from an inter-subspecies cross of Daguandao/IR28 by the single seed descent method was used to detect quantitative trait loci （QTLs） conferring resistance to rice false smut [caused by Ustilaginoidea virens （Cooke） Takahashi] in 2007 and 2009.The disease rate index of the two parents and 157 RILs caused by rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software.Seven QTLs controlling false smut resistance were detected on chromosomes 1,2,4,8,10,11,and 12,respectively,with the phenotypic variance of 9.8-22.5%.There were five and four QTLs detected in 2007 and 2009,respectively,and only two QTLs were found in both two years,the phenotypic variation was explained by individual QTL ranged from 18.0% to 19.3% for these two QTLs,and the additive effects of these two QTLs contributed to the 8.0-16.3% decrease of disease index and therefore the disease resistance increased.The direction of the additive effects at six loci qFsr1,qFsr2,qFsr8,qFsr10,qFsr11,and qFsr12 coincided with that predicted by phenotypes of the parents,and the IR28 alleles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao alleles at qFsr4.Both qFsr11 and qFsr12 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection （MAS） program.

关 键 词：水稻 稻曲病 抗病性 数量性状座位 

分 类 号：S511[农业科学—作物学]