比色法测定丙戊酸镁微丸的溶出度研究  

Determination of the dissolution of Magnesium valproate pellets by colorimetry

作　　者：薛云琴[1] 蒋曙光[1] 周建平[1] 

机构地区：[1]中国药科大学,江苏南京210009

出　　处：《安徽医药》2011年第5期559-561,共3页Anhui Medical and Pharmaceutical Journal

基　　金："重大新药创制"科技重大专项新制剂与新释药系统技术平台资助(No2009ZX09310-004)

摘　　要：目的采用比色法测定丙戊酸镁微丸的溶出度。方法利用镁离子与偶氮氯膦Ⅰ形成稳定的络合物,确定适宜的测定条件,建立丙戊酸镁体外测定方法。结果镁离子与偶氮氯膦Ⅰ在pH=10.0的氨性缓冲液环境中,形成稳定的紫红色络合物,该络合物在574 nm波长处有最大吸收。供试品中镁离子浓度在2.5～25.0 mg.L-1范围内与吸收度线性关系良好,r=0.999 7,精密度RSD<5.0%,平均回收率为100.4%～101.9%。结论该方法灵敏、准确、操作简便,可以作为丙戊酸镁微丸溶出度的检测方法。Aim To establish a method of colorimetry to determine the dissolution of Magnesium valproate pellets.Methods Mg2＋ and chlorophosphonazo-I formed stable purplish red complex with the determination of appropriate conditions,and the in vitro analytical methodology of Magnesium valproate was established.Results In pH 10 ammonia buffer solution,reagent CPA-I and Mg2＋ formed a stable purplish red complex with the maximum absorption at 574 nm.The linear range for Mg2＋ was 2.5~25.0 mg·L-1（r=0.999 7）,with the precision of RSD5% and average recovery of 100.4%~101.9%.Conclusion The colorimetric method is sensitive,accurate and easy to operate.It could be used for detecting the dissolution of Magnesium valproate pellets.

关 键 词：丙戊酸镁 微丸 溶出度 偶氮氯膦Ⅰ 比色法 

分 类 号：R927.2[医药卫生—药学]