活性艳蓝KN-R与DNA相互作用的光谱研究  

A Study on Spectra of Interaction between Reactive Brilliant Blue KN-R and DNA

作　　者：张艳霞 胥丹平[2] 张朝红[2] 铁梅[2] 王杰[2] 季晓彤[2] 李芳轶[2] 徐瑶[2] 

机构地区：[1]华北石油水电学校,任丘062552 [2]辽宁大学环境学院,沈阳11003

出　　处：《染料与染色》2011年第2期59-62,共4页Dyestuffs and Coloration

基　　金：辽宁省教育厅自然科学基金(L2010156)

摘　　要：采用紫外光谱和荧光光谱法研究了活性艳蓝KN-R(RBB KN-R)与DNA的相互作用。结果表明,RBBKN-R与DNA能够发生相互作用,其作用方式和程度取决于RBB KN-R浓度、离子强度、酸度和磷酸根浓度等因素。随着RBB KN-R浓度的增加,RBB KN-R-DNA的紫外光谱表现为增色效应,荧光发生猝灭。RBB KN-R主要与DNA中碱基基团结合,使DNA分子的双螺旋结构改变。NaCl加入可使DNA分子双螺旋结构轴向收缩。酸性条件时RBB KN-R主要与DNA的碱基基团作用;碱性条件时则抑制DNA与EB的结合。磷酸根离子的加入削弱了RBB KN-R对DNA的破坏强度。The interaction between Reactive Brilliant Blue KN-R（RBB KN-R）and DNA was studied by UV-vis and fluorescence spectra.The results showed that RBB KN-R could interact with DNA.The binding mode and effect degree depended on the concentration of RBB KN-R,ionic strength,solution acidity（pH） and phosphate concentration.With the increase of the concentration of RBB KN-R,hyperchromic effect was observed in UV-vis spectrum and fluorescence quenching phenomenon was observed in fluorescence spectrum.RBB KN-R was mainly bound to the base groups of DNA,which led to the change of DNA double-helix structure.The addition of NaCl caused in the axial shrinkage of DNA double-helix structure.In acidic condition,RBB KN-R mainly interacted with the base groups of DNA,but the bound of DNA and EB was restrained in alkaline condition.When the phosphate ions were added,the strength of DNA breaking by RBB KN-R was weakened.

关 键 词：活性艳蓝 脱氧核糖核酸 DNA 相互作用 

分 类 号：TQ615.5[化学工程—精细化工]