线粒体内膜KATP通道开放剂合用Na＋／Ca2＋交换阻滞剂对离体大鼠心肌梗死面积的影响  

Effect of Mitochondrial Inner KATP Channel Opener Combined with Na＋/Ca2＋ Exchanging Blocker on Myocardial Infarct Size in Isolated Rat Hearts

作　　者：贾大林[1] 魏敏杰[2] 

机构地区：[1]中国医科大学附属第一医院心血管内科,辽宁沈阳110001 [2]中国医科大学药学院药理学教研室,辽宁沈阳110001

出　　处：《医学临床研究》2011年第4期603-605,共3页Journal of Clinical Research

基　　金：[基金项目]辽宁省科技厅科学技术研究基金（No.2009225051-1）

摘　　要：【目的】探讨线粒体ATP敏感性钾通道（mitoKATP通道）开放剂尼可地尔合用Na＋／Ca2＋交换阻滞剂K昏R7943对离体大鼠心肌梗死面积的影响。【方法】离体大鼠心脏应用改良的Langendorff装置灌流，行45min缺血（即将左、右冠脉灌流量减到原灌流量的5Voo）及2h再灌注。Wistar大鼠40只，随机分为4组：对照组、尼可地尔组、K＆R7943组、尼可地尔合用K昏R7943组（合用药物组）。观察各组冠脉灌流液中超氧化物歧化酶（SOD）、丙二醛（MDA）的变化，心肌梗死面积的区别。【结果】对照组心肌梗死范围为34．31％，尼可地尔组为26．35％，KBR7943组为28．74％，两者合用时心肌梗死范围为19．23％，合用组显著低于其他三组（P〈0．01）。合用药物组SOD活性显著高于其它三组，MDA显著低于其他三组（P〈0．01）。【结论】尼可地尔合用KBR7943能明显减少心肌梗死面积，能提高冠脉灌流液中SOD活性，减少MDA含量。[Objective] To explore the effect of mitochondrial ATP-sensitive potassium channel （mitOKATp channel） opener nicorandil together with Na＋/Ca2＋ exchanging blocker KB-R7943 on myocardial infarct size in isolated rat hearts. [Methods]Isolated rat heart was perfused with modified Langendorff device and underwent the ischemia for 45min which left and right coronary perfusion volume reduced to 5 % of original perfusion volume, and then received reperfusion for 2min. Forty Wistar rats were randomly divided into control group, nic- orandil group, KB-R7943 group and nicorandil combined with KB-R7943 group（drug combination group）. The changes of superoxide dismutase（SOD） and malondialdehyde（MDA） in coronary perfusion fluid and the differ- ence of myocardial infarct size among the 4 groups were observed. [Results] Myocardial infarct size was 34.31% in control group, 26. 35% in nicorandil group, 28.74% in KB-R7943 group and 19. 23% in drug combination group. Myocardial infarct size in the drug combination group was significantly lower than that in other 3 groups（ P 〈0.01）. The SOD activity in the drug combination group was significantly higher than that in other 3 groups, hut MDA in the drug combination group was significantly lower than that in other 3 groups （P〈0.01）. [Conclusion] The combination of nicorandil and KB-R7943 can significantly reduce myocardial infarct size, and increase the SOD activity in coronary perfusion fluid, and decrease MDA levels.

关 键 词：心肌梗塞 钾通道 离子交换 离子通道 大鼠 

分 类 号：R542.22[医药卫生—心血管疾病]