Spatial heterogeneity of a microbial community in Kongsfjorden,Svalbard during late summer 2006 and its relationship to biotic and abiotic factors  

作　　者：GUO ChaoYing HE JianFeng ZHANG Fang CAI MingHong WANG GuiZhong 

机构地区：[1]Department of Oceanography, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China [2]SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China

出　　处：《Chinese Journal of Polar Science》2011年第1期55-66,共12页极地研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.41076130, 40576002);the International Cooperation Project of Science and Technology Commission of Shanghai Municipality (Grant no. 052307053);the Youth Marine Science Foundation of SOA (Grant no. 2010116);the Open Research Foundation of the SOA Laboratory of Marine Ecosystem and Biogeochemistry (Grant no. LMEB200902)

摘　　要：The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments （temperature, salinity, silicate, phosphate and nitrate, p 〈0.05） of the inner and outer basins of Kongsfjorden （Spitsbergen, Arctic） were studied using polymerase chain reaction-denaturing gradient gel electrophoresis（DGGE） fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria：Proteobacteria（Alpha, Beta and Gamma）, Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m （outer fjord） was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments （temperature, salinity, silicate, phosphate and nitrate, p 〈0.05） of the inner and outer basins of Kongsfjorden （Spitsbergen, Arctic） were studied using polymerase chain reaction-denaturing gradient gel electrophoresis（DGGE） fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria：Proteobacteria（Alpha, Beta and Gamma）, Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m （outer fjord） was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.

关 键 词：Microbial community Genetic diversity KONGSFJORDEN Arctic fjord 

分 类 号：S763.1[农业科学—森林保护学] X703[农业科学—林学]