葡萄球菌对克林霉素诱导性耐药的检测及分析  

Test and analysis of drug resistance of Staphylococcus against induced clindamycin

作　　者：李红凌[1] 罗湘蓉[1] 陈俐[1] 

机构地区：[1]贵州省人民医院中心实验室,贵阳550002

出　　处：《检验医学与临床》2011年第9期1037-1038,共2页Laboratory Medicine and Clinic

摘　　要：目的了解贵州省人民医院葡萄球菌临床分离株对红霉素和克林霉素的耐药性,检测红霉素对克林霉素诱导性耐药的发生率。方法用K-B法检测葡萄球菌对红霉素和克林霉素的耐药性,用D-试验检测克林霉素诱导性耐药。结果 165株葡萄球菌D-试验阳性率为14.6%(24/165)。对红霉素耐药同时对克林霉素敏感的葡萄球菌52株,D-试验阳性率为46.1%(24/52);红霉素和克林霉素均敏感的葡萄球菌为24株,D-试验均为阴性;红霉素和克林霉素均耐药的葡萄球菌89株,D-试验均阴性;D-试验阳性菌株分布在耐甲氧西林金黄色葡萄球菌(MR-SA)、甲氧西林敏感金黄色葡萄球菌(MSSA)、耐甲氧西林的凝固酶阴性葡萄球菌(MRSCNS)、耐甲氧西林敏感的凝固酶阴性葡萄球菌(MSSCNS)菌株中,阳性率分别为:MRSA为12.5%(5/40),MSSA为6.67%(1/15),MRSCNS为14.6%(15/103),MSSCNS为42.8%(3/7)。结论对表型为红霉素耐药同时对克林霉素敏感的葡萄球菌,应进行D-试验,检测克林霉素的诱导性耐药,避免不适当使用克林霉素导致临床上的治疗失败。Objective To understand the inducible drug resistance of Staphylococcus to erythromycin and clindamycin,and to analyze the occurrence rate of drug resistance of Staphylococcus to erythromycin and clindamycin.Methods The inducible drug resistance of Staphylococcus against erythromycin and clindamycin was determined by Kirby-Bauer discagar difusion test,and the inducible resistance of drug resistance against clindamycin was determined by D-test according to the standards of CLSI 2008（M100-S18）.Results The positive rate of D-test in 165 stains Staphylococcus was 15.55%（24/165）.The positive rate of D-test was 46.15%（24/52）in 52 stains of Staphylococcus resistant to erythromycin and sensistive to clindamycin.The D-test positive rate is zero（0/89） in 89 stains of Staphylococcus resistant to erythromycin and clindamycin,The D-test positive rate is zero（0/24） in 24 stains of Staphylococcus sensitive to erythromycin and clindamycin.The D-test positive stains exsit in MRSA,MSSA,MRCNS and MSCNS.Conclusion The inducible drug resistance of Staphylococcus against erythromycin and clindamycin should be tested by D-test.Staphylococcus drug resistance to erythromycin and sensistive to clindamycin should be tested in order to avoid clinicaltreament of Staphylococcus infection.

关 键 词：葡萄球菌 红霉素 克林霉素 诱导性耐药 

分 类 号：R378.11[医药卫生—病原生物学]