微波辅助衍生化-气相色谱法测定烟草中非挥发性有机酸  

GC Determination of Non-volatile Organic Acids in Tobacco with Microwave Assisted Derivatization

作　　者：蒋次清 胡守毅 李忠[1] 王璐[1] 王岚[1] 廖臻[1] 

机构地区：[1]云南烟草科学研究院云南省烟草化学重点实验室,昆明650106

出　　处：《理化检验（化学分册）》2011年第4期392-394,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：云南中烟工业公司基金项目(2008SB01)

摘　　要：在气相色谱法测定烟草中非挥发性有机酸中,提出了用硫酸-甲醇(1+7)混合溶液作衍生试剂,在微波炉中于选定的条件下对所测定的有机酸进行衍生化。经酯化的溶液用二氯甲烷萃取,萃取液蒸缩至2mL,分取1μL进样作气相色谱分析。测定中采用AC 20毛细管柱及火焰离子化(FID)检测器并用戊二酸作内标,对7种非挥发性有机酸用标准加入法作回收及精密度试验,测得回收率在97.5%～103.3%之间,相对标准偏差(n=5)在1.6%～4.4%之间。用所提出的方法测定了烤烟、卷烟及白肋烟样品中非挥发性有机酸,测定结果与文献方法的结果相符。In the gas chromatographic determination of non-volatile ogranic acids in tobacco,a modification of the derivatization of the organic acid was made by reacting with a mixture of H_2SO_4 and CH_3OH（1＋7）in a microoven under optimized working condition.The esterized solution was extracted with CH_2Cl_2,and the extract was evaporated to a volume of 2 mL.OneμL aliquot was taken for GC analysis.AC 20 capillary column and FID detector were used in the analysis,and pentanedioic acid was used as internal standard.Recovery and precision for 7 nonvolatile organic acids were tested by standanl addition method,values of recovery and RSD＇s（n=5）found were ranged from 97.5%to 103.3%and from 1.6%to 4.4%respectively.The proposed method was used in the determination of non-volatile organic acids in samples of flue-cured tobacco,cigarettes and burley tobacco,giving results in consistency with the results obtained by the method reported in literature.

关 键 词：气相色谱法 微波辅助衍生化 烟草 非挥发有机酸 

分 类 号：O657.7[理学—分析化学]